Journal Article10.1021/JACS.5B08844
Glycodendrimersomes from Sequence-Defined Janus Glycodendrimers Reveal High Activity and Sensor Capacity for the Agglutination by Natural Variants of Human Lectins
Shaodong Zhang,Qi Xiao,Samuel E. Sherman,Adam Muncan,Andrea D. M. Ramos Vicente,Zhichun Wang,Daniel A. Hammer,Dewight Williams,Yingchao Chen,Darrin J. Pochan,Sabine Vértesy,Sabine André,Michael L. Klein,Hans-Joachim Gabius,Virgil Percec +14 more
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TL;DR: The applicability of Janus-GDs to dissect structure-activity relationships between programmable cell surface models and human lectins in a highly sensitive and physiologically relevant manner is illustrated.
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Abstract: A library of eight amphiphilic Janus glycodendrimers (Janus-GDs) presenting d-lactose (Lac) and a combination of Lac with up to eight methoxytriethoxy (3EO) units in a sequence-defined arrangement was synthesized via an iterative modular methodology. The length of the linker between Lac and the hydrophobic part of the Janus-GDs was also varied. Self-assembly by injection from THF solution into phosphate-buffered saline led to unilamellar, monodisperse glycodendrimersomes (GDSs) with dimensions predicted by Janus-GD concentration. These GDSs provided a toolbox to measure bioactivity profiles in agglutination assays with sugar-binding proteins (lectins). Three naturally occurring forms of the human adhesion/growth-regulatory lectin galectin-8, Gal-8S and Gal-8L, which differ by the length of linker connecting their two active domains, and a single amino acid mutant (F19Y), were used as probes to study activity and sensor capacity. Unpredictably, the sequence of Lac on the Janus-GDs was demonstrated to deter...
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Citations
Mimicking Complex Biological Membranes and Their Programmable Glycan Ligands with Dendrimersomes and Glycodendrimersomes
TL;DR: All aspects of the synthesis of Janus dendrimers and JGDs and their biological activity and applications after assembly in aqueous media are covered.
150
One-Component Multifunctional Sequence-Defined Ionizable Amphiphilic Janus Dendrimer Delivery Systems for mRNA.
Dapeng Zhang,Elena N. Atochina-Vasserman,Devendra S. Maurya,Ning Huang,Qi Xiao,Nathan Ona,Matthew Liu,Hamna Shahnawaz,Houping Ni,Kyunghee Kim,Margaret M. Billingsley,Darrin J. Pochan,Michael J. Mitchell,Drew Weissman,Virgil Percec +14 more
TL;DR: In this article, the authors developed a one-component multifunctional ionizable amphiphilic Janus dendrimer (IAJD) delivery system for mRNA that exhibits high activity at a low concentration of ionizable amines organized in a sequence-defined arrangement.
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Self-Sorting and Coassembly of Fluorinated, Hydrogenated, and Hybrid Janus Dendrimers into Dendrimersomes
Qi Xiao,Jack D. Rubien,Zhichun Wang,Ellen H. Reed,Daniel A. Hammer,Dipankar Sahoo,Paul A. Heiney,Srujana S. Yadavalli,Mark Goulian,Samantha E. Wilner,Tobias Baumgart,Sergei A. Vinogradov,Michael L. Klein,Virgil Percec +13 more
TL;DR: The modular synthesis of a library containing seven self-assembling amphiphilic Janus dendrimers is reported, and their similar thicknesses to biological membranes by simple injection from ethanol solution into water or buffer make them important tools for synthetic biology.
Sweet complementarity: the functional pairing of glycans with lectins
TL;DR: The emerging evidence for the concept of a network calls for a detailed fingerprinting of glycans, and the validity of extrapolations between different organisms of the phylogenetic tree yet is inevitably limited.
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Exploring functional pairing between surface glycoconjugates and human galectins using programmable glycodendrimersomes.
Qi Xiao,Anna-Kristin Ludwig,Cecilia Romanò,Irene Buzzacchera,Irene Buzzacchera,Samuel E. Sherman,M. Vetro,Sabine Vértesy,Herbert Kaltner,Ellen H. Reed,Martin Möller,Christopher J. Wilson,Daniel A. Hammer,Stefan Oscarson,Michael L. Klein,Hans-Joachim Gabius,Virgil Percec +16 more
TL;DR: How synthetic JGD-headgroup tailoring teamed with protein engineering and network assays can help explain how molecular matchmaking operates in the cellular context of glycan and lectin complexity is demonstrated.
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References
Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells
TL;DR: It is shown that exosomes contain both mRNA and microRNA, which can be delivered to another cell, and can be functional in this new location, and it is proposed that this RNA is called “exosomal shuttle RNA” (esRNA).
Extracellular vesicles: exosomes, microvesicles, and friends.
TL;DR: This review focuses on the characterization of EVs and on currently proposed mechanisms for their formation, targeting, and function.
7.4K
Drug Delivery Systems: Entering the Mainstream
TL;DR: There is considerable interest in exploiting the advantages of DDS for in vivo delivery of new drugs derived from proteomics or genomics research and for their use in ligand-targeted therapeutics.
4.6K
Extracellular vesicles: biology and emerging therapeutic opportunities
TL;DR: Recent progress in understanding extracellular vesicle biology and the role of extrace cellular vesicles in disease is reviewed, emerging therapeutic opportunities are discussed and the associated challenges are considered.
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