Journal Article10.1002/BIOF.1565
Glucorticoid‐induced obesity individuals have distinct signatures of the gut microbiome
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TL;DR: Gut microbiota profiles of patients undergoing long-term treatment with GCs are developed and dramatically decreased gut microbial diversity is found in individuals with GC-induced obesity.
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Abstract: The human intestine and its resident microbiota are known to play an essential role in the pathogenesis of primary obesity. However, a little is known of the gut microbiota profiles in individuals with obesity induced by glucocorticoid (GC) therapy. Here, we recruited 28 people with GC-induced obesity and 27 age- and gender-matched healthy controls, whose feces have been collected separately, to delineate the gut microbial characteristics in GC-induced obesity individuals. High-throughput Illumina sequencing targeting the V4 region of the 16S rRNA gene has been applied to analyze the structure of the intestinal microbiome. Gas chromatography was also used to measure levels of short-chain fatty acids (SCFAs), a subset of key gut microbial metabolites mainly produced by Bacteroidetes. We found dramatically decreased gut microbial diversity in individuals with GC-induced obesity. In addition, the bacterial communities of the GC-induced obesity group were enriched in Firmicutes (e.g., genus Streptococcus) and depleted in Bacteroidetes. Furthermore, SCFA content was decreased in GC-induced obesity status. Overall, this study conducted a case-control study with 55 participants in which we analyzed gut microbiota to develop intestinal microbial profiles of patients undergoing long-term treatment with GCs. Concomitantly, the level of SCFAs was also detected in those study participants.
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Citations
Characteristics of gut microbiota in people with obesity.
TL;DR: Overall, this study revealed differences in the gut microbiota between people with obesity and control subjects, providing novel target for the treatment of individuals with obesity.
Involvement of the Gut Microbiota and Barrier Function in Glucocorticoid-Induced Osteoporosis
Jonathan D. Schepper,Fraser L. Collins,Naiomy Deliz Rios-Arce,Ho Jun Kang,Laura Schaefer,Joseph D Gardinier,Ruma Raghuvanshi,Robert A. Quinn,Robert A. Britton,Narayanan Parameswaran,Laura R. McCabe +10 more
TL;DR: The gut is identified as a novel therapeutic target for preventing glucocorticoid‐induced osteoporosis (GIO) and it is identified that bone‐specific Wnt10b overexpression prevented GIO.
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Glucocorticoid-induced loss of beneficial gut bacterial extracellular vesicles is associated with the pathogenesis of osteonecrosis
Chun-Yuan Chen,Shan-Shan Rao,Tao Yue,Yi-Juan Tan,Hao Yin,Ling-Jiao Chen,Ming-Jie Luo,Zun Wang,Yi-Yi Wang,Chun-Gu Hong,Yu-Xuan Qian,Ze‐Hui He,Jianghua Liu,Fei Yang,Feiyu Huang,Siyuan Tang,Huiqing Xie +16 more
TL;DR: This study demonstrates that cohousing with healthy mice or colonization with GM from normal mice attenuates GC-induced ONFH, and suggests that the GM is involved in protecting the femoral head by transferring bacterial EVs, and that loss of L. animalis and its EVs is associated with the development of GC- induced ONFH.
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The effect of glucocorticoic administration on bacterial translocation : evidence for an acquired mucosal immunodeficient state
J. Alverdy,E. Aoys +1 more
- 01 Jan 1991
TL;DR: Examination of the effect of dexamethasone administration on mucosal immunity and bacterial adherence and IgA suggests that glucocorticoids may promote bacterial translocation by impairment of mucosal IgA synthesis.
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Multicenter assessment of microbial community profiling using 16S rRNA gene sequencing and shotgun metagenomic sequencing.
TL;DR: Wang et al. as discussed by the authors evaluated the comparability of sequencing results of 16S rRNA gene sequencing (16Ss)- and shotgun metagenomic sequencing (SMs)-based microbial community profiling in laboratories under routine conditions.
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