Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex

TL;DR: A clustered regularly interspaced short palindromic repeats-based transcriptional activation screen focused on human E3 ligases, with the goal of identifying E3 ligases that can facilitate heterobifunctional compound-mediated target degradation, identified a candidate proteolysis-targeting chimera (PROTAC), 22-SLF.

TL;DR: An efficient golden gate based method to assemble gRNAs linked by optimal Csy4 ribonuclease sequences to deliver up to 10 g RNAs as a single gRNA array transcript to augment the capability and accessibility of multiplexed genome engineering.

TL;DR: In this paper , a toolbox of epigenetic effectors and gRNAs was used for patient-specific treatment of hepatocellular carcinoma (HCC) patients.

TL;DR: Four especially salient applications of CRISPR/Cas9 are covered: testing the consequences of enhancer mutations, tagging genes and gene products for visualization in live cells, directly activating or repressing enhancers in vivo, and manipulating the epigenome.

TL;DR: It is shown that accurate gene-level abundance estimates are best obtained with large numbers of short single-end reads, and estimates of the relative frequencies of isoforms within single genes may be improved through the use of paired- end reads, depending on the number of possible splice forms for each gene.

TL;DR: This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.

TL;DR: The results indicate that large, annotated cell-line collections may help to enable preclinical stratification schemata for anticancer agents and the generation of genetic predictions of drug response in the preclinical setting and their incorporation into cancer clinical trial design could speed the emergence of ‘personalized’ therapeutic regimens.

TL;DR: A new version of the database, MSigDB 3.0, is reported, with over 6700 gene sets, a complete revision of the collection of canonical pathways and experimental signatures from publications, enhanced annotations and upgrades to the web site.