Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex

TL;DR: One aspect of genome editing, the CRISPR/Cas9 system, is introduced to highlight its potential to correct genetic mutations and explore its utility in clinical setting to enlighten health care providers about genome editing and incite them to take part of this vital debate.

TL;DR: Current and potential applications of the CRISPR/Cas9 technology in hPSCs and how they contribute to establish hPSC as a model of choice for studying human genetics are reviewed.

TL;DR: The growing number of CRISPR screens are reviewed to increase the understanding of host-virus interactions and to identify potential anti-viral strategies.

TL;DR: The Transgenic RNAi Project (TRiP), a Drosophila melanogaster functional genomics platform at Harvard Medical School, was initiated in 2008 to generate and distribute a genome-scale collection of RNA interference (RNAi) fly stocks, and this work presents an update on these libraries of publicly available RNAi and CRISPR fly stocks.

TL;DR: It is shown that accurate gene-level abundance estimates are best obtained with large numbers of short single-end reads, and estimates of the relative frequencies of isoforms within single genes may be improved through the use of paired- end reads, depending on the number of possible splice forms for each gene.

TL;DR: This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.

TL;DR: The results indicate that large, annotated cell-line collections may help to enable preclinical stratification schemata for anticancer agents and the generation of genetic predictions of drug response in the preclinical setting and their incorporation into cancer clinical trial design could speed the emergence of ‘personalized’ therapeutic regimens.

TL;DR: A new version of the database, MSigDB 3.0, is reported, with over 6700 gene sets, a complete revision of the collection of canonical pathways and experimental signatures from publications, enhanced annotations and upgrades to the web site.