Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex

TL;DR: Several applications of CRISPR/Cas9 to stem cells related to degenerative diseases and the challenges and future perspectives regarding the use of this new technology as an important technology in the medical sciences are addressed.

TL;DR: The challenges facing in vivo epigenome editing and new strategies to overcome the huddles are discussed, and the dCas9 conjugation strategies to recruit endogenous chromatin modifiers and remodelers to specific genomic loci are discussed.

TL;DR: The results not only provide the feasibility of a reporter system for CRISPR library screening but also demonstrate the previously uncharacterized factor RMST as an important player in the modulation of DNA methylation.

TL;DR: CrisPR-RT (CRISPR RNA-Targeting), the first web application to help biologists design crRNAs with improved target specificity for the CRISPR-C2c2 system, is described, which will empower researchers in RNA editing.

TL;DR: It is shown that accurate gene-level abundance estimates are best obtained with large numbers of short single-end reads, and estimates of the relative frequencies of isoforms within single genes may be improved through the use of paired- end reads, depending on the number of possible splice forms for each gene.

TL;DR: This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.

TL;DR: The results indicate that large, annotated cell-line collections may help to enable preclinical stratification schemata for anticancer agents and the generation of genetic predictions of drug response in the preclinical setting and their incorporation into cancer clinical trial design could speed the emergence of ‘personalized’ therapeutic regimens.

TL;DR: A new version of the database, MSigDB 3.0, is reported, with over 6700 gene sets, a complete revision of the collection of canonical pathways and experimental signatures from publications, enhanced annotations and upgrades to the web site.