Journal Article10.1002/STEM.5530120404
Gene therapy utilizing drug resistance genes: A review
Debabrata Banerjee,Shi Cheng Zhao,Ming‐Xia ‐X Li,Barry I. Schweitzer,Shin Mineishi,Joseph R. Bertino +5 more
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TL;DR: Animal models and in vitro work with human progenitors using drug resistance genes are reviewed, and the generation of drug resistant bone marrow may facilitate the development of aggressive chemotherapeutic regimens that might otherwise be lethal due to marrow toxicity.
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Abstract: The generation of drug resistant bone marrow may facilitate the development of aggressive chemotherapeutic regimens that might otherwise be lethal due to marrow toxicity. With the availability of technology that permits in vitro manipulation of human marrow and peripheral blood stem cells, it is now possible to introduce genes that confer drug resistance to these hematopoietic progenitors. Animal models and in vitro work with human progenitors using drug resistance genes are reviewed.
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Citations
Novel aspects of resistance to drugs targeted to dihydrofolate reductase and thymidylate synthase.
Debabrata Banerjee,Philipp Mayer-Kuckuk,Gina M. Capiaux,Gina M. Capiaux,Tulin Budak-Alpdogan,Richard Gorlick,Joseph R. Bertino +6 more
TL;DR: The molecular mechanisms that govern translational regulation of DHFR in response to MTX as an additional resistance mechanism are studied in detail and gene copy number of the E2F-1 gene appears to be increased in a significant number of samples obtained from metastases of colorectal cancer.
269
Engraftment of MDR1 and NeoR gene-transduced hematopoietic cells after breast cancer chemotherapy.
Jeffrey A. Moscow,Hui Huang,Hui Huang,Charles Carter,Charles Carter,Kenneth Hines,Kenneth Hines,Jo Anne Zujewski,Jo Anne Zujewski,Georgie Cusack,Georgie Cusack,Cathy Chow,Cathy Chow,David Venzon,David Venzon,Brian Sorrentino,Brian Sorrentino,Yawen Chiang,Yawen Chiang,Barry Goldspiel,Barry Goldspiel,Susan F. Leitman,Susan F. Leitman,Elizabeth J. Read,Elizabeth J. Read,Andrea Abati,Andrea Abati,Michael M. Gottesman,Michael M. Gottesman,Ira Pastan,Ira Pastan,Stephanie Sellers,Stephanie Sellers,Cynthia E. Dunbar,Cynthia E. Dunbar,Kenneth H. Cowan +35 more
TL;DR: It is found that hematopoietic reconstitution can occur using only PBPCs incubated ex vivo, that the MDR1 gene product may play a role in engraftment, and that chemotherapy may selectively expand MDR 1 gene-transduced hematoplastic cells relative to NeoR transduced cells in some patients.
135
Improving enzymes for cancer gene therapy
TL;DR: This review considers genes for the ablation of tumors, namely, genes that have been mutated to afford enhanced activation of prodrugs and increased sensitization of tumors to specific chemotherapeutic agents.
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•Journal Article
Active site-directed double mutants of dihydrofolate reductase
Emine A. Ercikan-Abali,Shin Mineishi,Youzhi Tong,Saori Nakahara,Mark Waltham,Debabrata Banerjee,Wen Chen,Michel Sadelain,Joseph R. Bertino +8 more
TL;DR: Comparison of in vitro measured properties with those of single-residue variants indicates that double mutants are indeed significantly superior, and this was verified for one of the double mutants that provided high-level methotrexate resistance following retrovirus-mediated gene transfer in NIH3T3 cells.
Molecular mechanisms of resistance to antifolates, a review.
Debabrata Banerjee,Emine A. Ercikan-Abali,Mark Waltham,Barbara Schnieders,Daniel Hochhauser,Wei Wei Li,Jianguo Fan,Richard Gorlick,Erdem Goker,Joseph R. Bertino +9 more
TL;DR: Cloning of the human folylpolyglutamate synthase gene and the reduced folate transport gene have been reported recently and should facilitate the identification of the molecular basis of these resistant phenotypes.
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