Gene replacement and retrieval with recombinant M13mp bacteriophages.
62
TL;DR: An allele exchange system for shuttling sequences of DNA to and from their original chromosomal loci by exploiting unique properties of M13 lysogens to devise positive selections to detect integration and excision and manipulate phenotypically silent alleles.
read more
Abstract: We have developed an allele exchange system for shuttling sequences of DNA to and from their original chromosomal loci. Cloned segments of the histidine operon of Salmonella typhimurium and the lactose operon of Escherichia coli served as target sequences and were used to develop the system. Replacement and retrieval of target sequences used the phage M13mp vectors and proceeded through an M13 lysogen intermediate. The intermediates and products of allele exchange were characterized by genetic and hybridization analyses. Several unique properties of M13 lysogens were exploited to devise positive selections to detect integration and excision. These positive selections were used to manipulate phenotypically silent alleles.
read more
Chat with Paper
AI Agents for this Paper
Find similar papers on Google Scholar, PubMed and Arxiv
Write a critical review of this paper
Analyze citations of this paper to find unaddressed research gaps
Citations
Regulation of Escherichia coli topA gene transcription: involvement of a sigmaS-dependent promoter.
TL;DR: The activity of promoter P1 on the chromosome increased during heat shock, consistent with the previous result obtained using the topA-galK fusion plasmid showing that P1 is a sigma32-dependent heat shock promoter.
43
Chromosomal domains of supercoiling in Salmonella typhimurium.
TL;DR: The level of supercoiling does not differ significantly between chromosomal domains, and that each domain responds in a similar fashion to factors that perturb supercoiled, have implications for the organization of the bacterial genome.
43
New method for gene disruption in Salmonella typhimurium: construction and characterization of an ada-deletion derivative of Salmonella typhimurium TA1535.
TL;DR: A new method for gene disruption in Salmonella typhimurium was developed and used to construct a mutant in which the resident ada-like (adaST) gene was replaced with a kanamycin resistance gene to produce an adaST-deletion mutant derivative.
40
Bacterial Growth State Distinguished by Single-Cell Protein Profiling: Does Chlorination Kill Coliforms in Municipal Effluent?
TL;DR: The results indicate that chlorination failed to kill coliforms but rather elicited nutrient starvation and a reversible nonculturable state, suggesting that the current standard procedures for wastewater analysis which rely on detection of culturable cells likely underestimate fecal coliform content.
Aberrant Cell Division and Random FtsZ Ring Positioning in Escherichia coli cpxA* Mutants
Joe Pogliano,Jian Ming Dong,Peter De Wulf,Dierdre Furlong,Dana Boyd,Richard Losick,Kit Pogliano,Edmund C. C. Lin +7 more
TL;DR: It appears that certain mutant sensor kinases (CpxA*) either exhibit hyperactivity on CpxR or extend their signalling activity to one or more noncognate response regulators involved in cell division.
34
References
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
TL;DR: This paper describes a method of transferring fragments of DNA from agarose gels to cellulose nitrate filters that can be hybridized to radioactive RNA and hybrids detected by radioautography or fluorography.
33.7K
•Book
Experiments in molecular genetics
Jeffrey H Miller
- 01 Jan 1972
TL;DR: Molecular Genetics (Biology): An Overview | Sciencing Experimental in Molecular Genetics Experiments in molecular genetics (1972 edition) | Open ...
29.6K
Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors
TL;DR: New Escherichia coli host strains have been constructed for the E. coli bacteriophage M13 and the high-copy-number pUC-plasmid cloning vectors and mutations introduced into these strains improve cloning of unmodified DNA and of repetitive sequences.
15.9K
Studies on transformation of Escherichia coli with plasmids
Douglas Hanahan,Douglas Hanahan +1 more
TL;DR: Competition with both transforming and non-transforming plasmids indicates that each cell is capable of taking up many DNA molecules, and that the establishment of a transformation event is neither helped nor hindered significantly by the presence of multiple plasmid molecules.
12.1K
Related Papers (5)
Jeffrey H Miller
- 01 Jan 1972
Thomas J. Silhavy,Michael L. Berman,Lynn W. Enquist +2 more
- 01 Jul 1984