Fusion protein constructs

TL;DR: In this article, cell-free systems for generating carbapenems, e.g., a compound of the Formula (I): or salts thereof; wherein (II), R1, R2, R3, R4, R5, and R6 are defined.

TL;DR: In this paper, the authors describe methods for preparing cells that can be used as biocatalysts by altering enzymes that compete for a substrate or product of a pathway of interest such that the targeted enzyme is sensitive to a site-specific protease, which protease is expressed but relocated in the cell to another site where it is not in contact with the target enzyme in the intact cell.

TL;DR: In this article, a method for preparing cells that can be used as biocatalysts by inducing in them a growth-decoupled state, in which interferase inhibits the expression of genes except the ones that code for the pathway enzymes of interest.

TL;DR: In this paper, biological macromolecules are synthesized in vitro under conditions and in a reaction composition wherein oxidative phosphorylation is activated and protein folding is improved, and the results show that protein folding can be improved.

TL;DR: In this article, methods for the utilization of bacterial cell-free extracts in the synthesis of polypeptides containing unnatural amino acids at one or more specified residues of the polyprotein were provided.

TL;DR: Biological macromolecules are synthesized in vitro in a thin film that provides for high surface area/volume ratio, allowing improved yield in scaled up reactions as mentioned in this paper. But their performance is limited.

TL;DR: In this paper, the authors presented a method for the improved in vitro synthesis of polypeptides, where the duration of detectable protein synthesis in a reaction is substantially extended over existing methods, thereby providing for increased total yield of polyptide.