Open AccessJournal Article
Expression profiles during dedifferentiation in newt lens regeneration revealed by expressed sequence tags.
Nobuyasu Maki,John Martinson,Osamu Nishimura,Hiroshi Tarui,Jaroslaw Meller,Panagiotis A. Tsonis,Kiyokazu Agata +6 more
TL;DR: The expression sequence tag (EST) resource is useful for the future study of newt dedifferentiation, and the sequence information is available in GenBank (accession numbers; FS290155-FS300559).
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Abstract: Purpose: The adult newt can regenerate lens from pigmented epithelial cells (PECs) of the dorsal iris via dedifferentiation. The purpose of this research is to obtain sequence resources for a newt lens regeneration study and to obtain insights of dedifferentiation at the molecular level. Methods: mRNA was purified from iris during dedifferentiation and its cDNA library was constructed. From the cDNA library 10,449 clones were sequenced and analyzed. Results: From 10,449 reads, 780 contigs and 1,666 singlets were annotated. The presence of several cancer- and apoptosisrelated genes during newt dedifferentiation was revealed. Moreover, several candidate genes, which might participate in reprogramming during dedifferentiation, were also found. Conclusions: The expression of cancer- and apoptosis-related genes could be hallmarks during dedifferentiation. The expression sequence tag (EST) resource is useful for the future study of newt dedifferentiation, and the sequence information is available in GenBank (accession numbers; FS290155-FS300559).
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Citations
Dedifferentiation, transdifferentiation and reprogramming: three routes to regeneration.
TL;DR: Current research aims to understand how the processes of dedifferentiation, transdifferentiation or reprogramming work and to eventually harness them for use in regenerative medicine.
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TL;DR: A perspective is looked forward in a perspective to discuss the many unanswered questions raised by investigations of the past century, what new approaches can be taken to answer them, and what the prospects are for translation of basic research on limb regeneration into clinical means to regenerate human appendages.
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TL;DR: Higher level cognitive processing would likely be required for receivers and senders to make potentially informed decisions that reflect tradeoffs in their survival and fitness.
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Efficient regeneration by activation of neurogenesis in homeostatically quiescent regions of the adult vertebrate brain.
Daniel A. Berg,Matthew Kirkham,Anna Beljajeva,Dunja Knapp,Bianca Habermann,Jesper Ryge,Elly M. Tanaka,András Simon +7 more
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Defining a large set of full-length clones from a Xenopus tropicalis EST project
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From biomedicine to natural history research: EST resources for ambystomatid salamanders
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Hmgb3: an HMG-box family member expressed in primitive hematopoietic cells that inhibits myeloid and B-cell differentiation
Michael J. Nemeth,David J. Curtis,Martha Kirby,Lisa Garrett-Beal,Nancy E. Seidel,Amanda P. Cline,David M. Bodine +6 more
TL;DR: It is concluded that down-regulation of Hmgb3 protein levels is an important step for myeloid and B-cell differentiation.
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Regulation of lens regeneration by fibroblast growth factor receptor 1.
TL;DR: This paper shows that FGFR‐1 expression and function is correlated with the process of lens regeneration from the dorsal iris, and shows that an FG FR‐1‐specific inhibitor is able to inhibit theprocess of transdifferentiation and lens regeneration.
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