Journal Article10.1007/S12010-007-0003-X
Expression of recombinant proteins in Pichia pastoris.
Pingzuo Li,Pingzuo Li,Anukanth Anumanthan,Xiu Gong Gao,Kuppusamy Ilangovan,Vincent Suzara,Nejat Düzgüneş,V. Renugopalakrishnan,V. Renugopalakrishnan,V. Renugopalakrishnan +9 more
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TL;DR: This review discusses and evaluates the recent progress in optimizing the fermentation process to improve the yield and stability of expressed proteins and the glycosylation profile of Pichia pastoris.
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Abstract: Pichia pastoris has been used extensively and successfully to express recombinant proteins. In this review, we summarize the elements required for expressing heterologous proteins, and discuss various factors in applying this system for protein expression. These elements include vectors, host strains, heterologous gene integration into the genome, secretion factors, and the glycosylation profile. In particular, we discuss and evaluate the recent progress in optimizing the fermentation process to improve the yield and stability of expressed proteins. Optimization can be achieved by controlling the medium composition, pH, temperature, and dissolved oxygen, as well as by methanol induction and feed mode.
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Citations
•Journal Article
Methods in Enzymology.
TL;DR: This volume is keyed to high resolution electron microscopy, which is a sophisticated form of structural analysis, but really morphology in a modern guise, the physical and mechanical background of the instrument and its ancillary tools are simply and well presented.
3.6K
Pichia pastoris: A highly successful expression system for optimal synthesis of heterologous proteins
TL;DR: P. pastoris expression system is one of the most popular and standard tools for the production of recombinant protein in molecular biology and is also considered a unique host for the expression of subunit vaccines which could significantly affect the growing market of medical biotechnology.
482
Cultivation strategies to enhance productivity of Pichia pastoris: A review
Verena Looser,Béla Brühlmann,Fabian Bumbak,Chantal Stenger,Merixtell Costa,Andrea Camattari,Dimitrios Fotiadis,Karin Kovar +7 more
TL;DR: This review focuses on particular aspects of recombinant protein production related to variations in biomass growth and their implications for strain design and screening, as well as on the concept of rational comparisons between cultivation systems for the development of specific production processes in bioreactors.
327
Deletion of the Pichia pastoris KU70 homologue facilitates platform strain generation for gene expression and synthetic biology.
Laura Näätsaari,Beate Mistlberger,Claudia Ruth,Tanja Hajek,Franz Stefan Hartner,Anton Glieder +5 more
TL;DR: The use of the ku70 deletion strain for fast and simple screening of genes in the search of new auxotrophic markers by targeting dihydroxyacetone synthase and glycerol kinase genes will further support protein production strain generation and synthetic biology using P. pastoris strains as platform hosts.
References
•Journal Article
Methods in Enzymology.
TL;DR: This volume is keyed to high resolution electron microscopy, which is a sophisticated form of structural analysis, but really morphology in a modern guise, the physical and mechanical background of the instrument and its ancillary tools are simply and well presented.
3.6K
Heterologous protein expression in the methylotrophic yeast Pichia pastoris
TL;DR: This paper reviews the P. pastoris expression system: how it was developed, how it works, and what proteins have been produced and describes new promoters and auxotrophic marker/host strain combinations which extend the usefulness of the system.
2.2K
Strategies for achieving high-level expression of genes in Escherichia coli.
TL;DR: Progress in the understanding of several biological processes promises to broaden the usefulness of Escherichia coli as a tool for gene expression and the remarkable increase in the availability of fusion partners offers a wide range of tools for improved protein folding, solubility, protection from proteases, yield, and secretion into the culture medium.
1.3K
Recent advances in the expression of foreign genes in Pichia pastoris
TL;DR: The Pichia pastoris heterologous gene expression system has been utilized to produce attractive levels of a variety of intracellular and extracellular proteins of interest and improvements in understanding and application have improved its utility even further.
1K
Production of recombinant proteins in fermenter cultures of the yeast Pichia pastoris.
TL;DR: The Pichia pastoris expression system offers economy, ease of manipulation, the ability to perform complex post-translational modifications, and high expression levels.
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