Exosome-Mediated Crosstalk between Keratinocytes and Macrophages in Cutaneous Wound Healing

TL;DR: Biomaterial-assisted exosomes accelerate skin wound repair by maintaining exosome bioactivity and providing a conducive microenvironment, with recent advances in exosome classification and engineering methods enhancing their therapeutic potential for chronic wound treatment.

TL;DR: MVs derived from dermal myofibroblasts modify the integrity of the blood and lymphatic barriers using distinct endocytosis pathways. MVs cross the LEC barrier but not the BEC barrier, and influence cell-cell junctions in both BECs and LECs.

TL;DR: Fetal skin achieves scarless wound repair and the co-existence of an HGG fibroblast subset with FPR1high myeloid cells of fetal origin is identified indicating common underlying biological processes.

TL;DR: This Review suggests a new grouping of macrophages based on three different homeostatic activities — host defence, wound healing and immune regulation, and proposes that similarly to primary colours, these three basic macrophage populations can blend into various other 'shades' of activation.

TL;DR: A set of standards encompassing three principles-the source of macrophages, definition of the activators, and a consensus collection of markers to describe macrophage activation are described with the goal of unifying experimental standards for diverse experimental scenarios.

TL;DR: Exosomes were described as vesicles of endosomal origin secreted from reticulocytes in the 1980s as discussed by the authors, and their biogenesis, their secretion, and their subsequent fate are discussed, as their functions rely on these important processes.

TL;DR: A novel toolbox for subcellular colocalization analysis under ImageJ is created that integrates current global statistic methods and a novel object‐based approach to assess proteins residing on intracellular structures by fluorescence microscopy.