Journal Article10.3109/10520297009085351
Evaluation of Pollen Viability by Enzymatically Induced Fluorescence; Intracellular Hydrolysis of Fluorescein Diacetate
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TL;DR: The FCR provides an effective method for assessing pollen quality and is primarily one for the integrity of the plasmalemma of the vegetative cell, which is likely to be closely correlated with viability.
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Abstract: Viable pollen grains immersed in a solution of fluorescein diacetate, made up in a concentration of about 10−6 M in sucrose of suitable tonicity (usually about 0.5 M), rapidly accumulate free fluorescein, which can be detected by its fluorescence. This fluorochromatic reaction (FCR) probably depends upon the entry of the nonpolar substrate into the vegetative cell where it is hydrolyzed by esterase to give the polar product, fluorescein, which is retained by the cell membrane. The test is primarily one for the integrity of the plasmalemma of the vegetative cell. Since this integrity is likely to be closely correlated with viability, the FCR provides an effective method for assessing pollen quality.
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Citations
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TL;DR: The stigmas of buds of the age range capable of accepting self-pollen (bud pollination) in self incompatible individuals of Raphanus sativus and Cheiranthus cheiri were found to have cuticularised papillae already carrying a thin surface secretion over the receptive area, which reduced the germination rate of both compatible and incompatible pollen and prevented the tubes that were formed from penetrating the cuticle of the Papillae, so abolishing the self compatibility.
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Inheritance and linkage relationships of glutamate oxaloacetate transaminase isoenzymes in apple : 1. The gene GOT-1, a marker for the S incompatibility locus.
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References
Pollen-wall proteins: localization and enzymic activity.
R. B. Knox,J. S. Heslop-Harrison +1 more
TL;DR: In this paper, the authors examined the distribution of acid phosphatase, ribonuclease, esterase, amylase and protease activity and protein in the walls of pollen grains and spores.
209
Cytochemical Localization of Enzymes in the Wall of the Pollen Grain
R. B. Knox,J. S. Heslop-Harrison +1 more
TL;DR: The storage of various hydrolytic enzymes in the pollen walls of ten species of flowering plants is reported, and it is shown that the site of deposition is the cellulosic intine in walls of several different structural types.
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