Evaluation of Diagnostic Assay for Rickettsioses Using Duplex Real-Time PCR in Multiple Laboratories in Japan.
Fumihiko Kawamori,Y. Shimazu,Hiroko Sato,Naota Monma,Asaka Ikegaya,Seigo Yamamoto,Hiromi Fujita,Hiroshi Morita,Yukiko Tamaki,Naoya Takamoto,Hongru Su,Masahiko Shimada,Yuko Shimamura,Shuichi Masuda,Shuji Ando,Norio Ohashi +15 more
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TL;DR: The detection efficiency of this duplex real-time PCR assay for O. tsutsugamushi from blood or skin (eschar) specimens appeared to be almost the same as that of the conventional PCR method, even when performed in different laboratories, whereas the efficiency for spotted fever group rickettsiae tended to be higher than that ofThe 2 traditional double PCR assays.
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Abstract: Tsutsugamushi disease and Japanese spotted fever are representative rickettsioses in Japan, and are caused by infection with Orientia tsutsugamushi and Rickettsia japonica, respectively. For molecular-based diagnosis, conventional PCR assays, which independently amplify respective rickettsial DNA, are usually used; however, this approach is time-consuming. Here, we describe a new duplex real-time PCR assay for the simultaneous detection of O. tsutsugamushi and spotted fever group rickettsiae, and its evaluation using several PCR conditions in 6 public health laboratories. The detection limit of the assay was estimated to be 102 copies and the sensitivity was almost identical to that of 3 conventional PCR methods. A total of 317 febrile patients were selected as clinically suspected or confirmed cases of rickettsioses. The detection efficiency of this assay for O. tsutsugamushi from blood or skin (eschar) specimens appeared to be almost the same as that of the conventional PCR method, even when performed in different laboratories, whereas the efficiency for spotted fever group rickettsiae tended to be higher than that of the 2 traditional double PCR assays. Our duplex real-time PCR is thus a powerful tool for the rapid diagnosis of rickettsioses, especially at the acute stage of infection.
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Citations
Descriptive epidemiology of rickettsial infections in Japan: Scrub typhus and Japanese spotted fever, 2007-2016.
Hitomi Kinoshita,Yuzo Arima,Mika Shigematsu,Tomimasa Sunagawa,Masayuki Saijo,Kazunori Oishi,Shuji Ando +6 more
TL;DR: In this article, the epidemiological and clinical characteristics of two rickettsial diseases, scrub typhus (ST) and Japanese spotted fever (JSF), in Japan have been described.
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Molecular Detection of Tick-Borne Bacterial and Protozoan Pathogens in Haemaphysalis longicornis (Acari: Ixodidae) Ticks from Free-Ranging Domestic Sheep in Hebei Province, China
TL;DR: Wang et al. as mentioned in this paper collected 646 Ha. longicornis ticks from free-ranging domestic sheep in the southern region of Hebei Province, China, and detected tick-borne pathogens of zoonotic and veterinary importance (i.e., Rickettsia, Anaplasma, Ehrlichia, Borrelia, Theileria, and Hepatozoon spp.) using PCR assays and sequence analysis.
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The clinical course of 239 cases of Japanese spotted fever in Ise Red Cross Hospital, 2006–2019
TL;DR: In this article, a case fatality rate of 3.3% indicates that Japanese spotted fever is a severe illness, and early initiation of therapy was shown to improve JSF-induced mortality by approximately 1%.
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The clinical course of 239 cases of Japanese spotted fever in Ise Red Cross Hospital, 2006–2019
TL;DR: In this article , a case fatality rate of 3.3% indicates that Japanese spotted fever is a severe illness, and early initiation of therapy was shown to improve JSF-induced mortality by approximately 1%.
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Rickettsia japonica Infection after Land Leech Bite, Japan
TL;DR: A case of Rickettsia japonica infection in an 81-year-old man in central Japan had fever, rash, and an eschar but no evidence of a tick bite.
References
Isolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16S ribosomal RNA
TL;DR: Using a set of synthetic oligonucleotides homologous to broadly conserved sequences in-vitro amplification via the polymerase chain reaction followed by direct sequencing results in almost complete nucleotide determination of a gene coding for 16S ribosomal RNA.
2.8K
Rapid and simple determination of the Escherichia coli phylogenetic group.
TL;DR: In this paper, a simple and rapid phylogenetic grouping technique based on triplex PCR was proposed, which uses a combination of two genes (chuA and yjaA) and an anonymous DNA fragment, was tested with 230 strains and showed excellent correlation with reference methods.
2.7K
Dysregulation of Immune Response in Patients with COVID-19 in Wuhan, China
Chuan Qin,Luoqi Zhou,Ziwei Hu,Shuo-Qi Zhang,Sheng Yang,Yu Tao,Cuihong Xie,Ke Ma,Ke Shang,Wei Wang,Dai-Shi Tian +10 more
TL;DR: Patients with COVID-19 have lower level of regulatory T cells, and more obviously damaged in severe cases, compared with non-severe patients, which suggests surveillance of NLR and lymphocyte subsets is helpful in the early screening of critical illness, diagnosis and treatment of CO VID-19.
2.6K
Clinical progression and viral load in a community outbreak of coronavirus-associated SARS pneumonia: a prospective study.
Joseph S. M. Peiris,Chung-Ming Chu,V. C. C. Cheng,Kin Sang Chan,Ivan Hung,Leo L.M. Poon,K I Law,Bone S.F. Tang,T Y W Hon,CS Chan,Kwok-ning Chan,J S C Ng,Bo-Jian Zheng,Wing-Chun Ng,Raymond Lai,Yi Guan,Kwok-Yung Yuen +16 more
TL;DR: The consistent clinical progression, shifting radiological infiltrates, and an inverted V viral-load profile suggest that worsening in week 2 is unrelated to uncontrolled viral replication but may be related to immunopathological damage.
2.5K