Evaluation of Diagnostic Assay for Rickettsioses Using Duplex Real-Time PCR in Multiple Laboratories in Japan.
Fumihiko Kawamori,Y. Shimazu,Hiroko Sato,Naota Monma,Asaka Ikegaya,Seigo Yamamoto,Hiromi Fujita,Hiroshi Morita,Yukiko Tamaki,Naoya Takamoto,Hongru Su,Masahiko Shimada,Yuko Shimamura,Shuichi Masuda,Shuji Ando,Norio Ohashi +15 more
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TL;DR: The detection efficiency of this duplex real-time PCR assay for O. tsutsugamushi from blood or skin (eschar) specimens appeared to be almost the same as that of the conventional PCR method, even when performed in different laboratories, whereas the efficiency for spotted fever group rickettsiae tended to be higher than that ofThe 2 traditional double PCR assays.
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Abstract: Tsutsugamushi disease and Japanese spotted fever are representative rickettsioses in Japan, and are caused by infection with Orientia tsutsugamushi and Rickettsia japonica, respectively. For molecular-based diagnosis, conventional PCR assays, which independently amplify respective rickettsial DNA, are usually used; however, this approach is time-consuming. Here, we describe a new duplex real-time PCR assay for the simultaneous detection of O. tsutsugamushi and spotted fever group rickettsiae, and its evaluation using several PCR conditions in 6 public health laboratories. The detection limit of the assay was estimated to be 102 copies and the sensitivity was almost identical to that of 3 conventional PCR methods. A total of 317 febrile patients were selected as clinically suspected or confirmed cases of rickettsioses. The detection efficiency of this assay for O. tsutsugamushi from blood or skin (eschar) specimens appeared to be almost the same as that of the conventional PCR method, even when performed in different laboratories, whereas the efficiency for spotted fever group rickettsiae tended to be higher than that of the 2 traditional double PCR assays. Our duplex real-time PCR is thus a powerful tool for the rapid diagnosis of rickettsioses, especially at the acute stage of infection.
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Citations
Descriptive epidemiology of rickettsial infections in Japan: Scrub typhus and Japanese spotted fever, 2007-2016.
Hitomi Kinoshita,Yuzo Arima,Mika Shigematsu,Tomimasa Sunagawa,Masayuki Saijo,Kazunori Oishi,Shuji Ando +6 more
TL;DR: In this article, the epidemiological and clinical characteristics of two rickettsial diseases, scrub typhus (ST) and Japanese spotted fever (JSF), in Japan have been described.
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Molecular Detection of Tick-Borne Bacterial and Protozoan Pathogens in Haemaphysalis longicornis (Acari: Ixodidae) Ticks from Free-Ranging Domestic Sheep in Hebei Province, China
TL;DR: Wang et al. as mentioned in this paper collected 646 Ha. longicornis ticks from free-ranging domestic sheep in the southern region of Hebei Province, China, and detected tick-borne pathogens of zoonotic and veterinary importance (i.e., Rickettsia, Anaplasma, Ehrlichia, Borrelia, Theileria, and Hepatozoon spp.) using PCR assays and sequence analysis.
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The clinical course of 239 cases of Japanese spotted fever in Ise Red Cross Hospital, 2006–2019
TL;DR: In this article, a case fatality rate of 3.3% indicates that Japanese spotted fever is a severe illness, and early initiation of therapy was shown to improve JSF-induced mortality by approximately 1%.
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The clinical course of 239 cases of Japanese spotted fever in Ise Red Cross Hospital, 2006–2019
TL;DR: In this article , a case fatality rate of 3.3% indicates that Japanese spotted fever is a severe illness, and early initiation of therapy was shown to improve JSF-induced mortality by approximately 1%.
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Rickettsia japonica Infection after Land Leech Bite, Japan
TL;DR: A case of Rickettsia japonica infection in an 81-year-old man in central Japan had fever, rash, and an eschar but no evidence of a tick bite.
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