Open AccessDissertation
Étude des propriétés de surface du bactériophage MS2 et du norovirus murin au cours de différents traitements d’inactivation
Adrien Brié
- 25 Jan 2017
2
TL;DR: In this paper, a travail de these a pour objectif d'etudier l'evolution des proprietes de surface (charge and hydrophobie) de virus modeles, bacteriophage MS2 and norovirus murin, au cours de l'inactivation par la chaleur, l'hypochlorite de sodium and l'ozone.
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Abstract: Meme si les traitements thermiques ou la desinfection par les oxydants ont demontre leur efficacite virucide, les mecanismes lies a la perte du caractere infectieux ne sont pas connus. Ceci pose un reel probleme d’interpretation de la presence de genome viral en matiere de risque infectieux dans les aliments. Ce travail de these a pour objectif d’etudier l’evolution des proprietes de surface (charge et hydrophobie) de virus modeles, bacteriophage MS2 et norovirus murin, au cours de l’inactivation par la chaleur, l’hypochlorite de sodium et l’ozone. Pour nos deux virus, nous demontrons l’existence d’une temperature critique au-dela de laquelle la particule virale se destructure en liberant son genome. Un simple traitement a la RNase permettrait alors de ne detecter que des virus infectieux par biologie moleculaire. Le traitement thermique implique aussi une augmentation de l’hydrophobie soulignant des modifications conformationnelles de la capside. L’hypochlorite de sodium ne modifie que peu les proprietes de surface mais des phenomenes d’oxydation ont lieu au niveau de la capside puisque la charge du bacteriophage MS2 est legerement modifiee. Ces modifications diminuent la resistance thermique du virus. Nous demontrons un effet synergique de l’hypochlorite de sodium et la chaleur sur le bacteriophage MS2 (inactivation, RNase et hydrophobie). Quant a l’ozone gazeux, nous soulignons son interet pour le traitement virucide des aliments fragiles. Ainsi, ce travail precise les mecanismes d’inactivation des virus et ouvre de nouvelles perspectives tant pour discriminer les virus infectieux et non-infectieux que pour proposer l’exploration de nouveaux traitements technologiques
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Citations
Survival and Transfer of Murine Norovirus within a Hydroponic System during Kale and Mustard Microgreen Harvesting
Qing Wang
- 28 Jul 2015
TL;DR: It is seen that viruses can be recirculated in water, even after an initial contamination event is removed, taken up through the roots of microgreens, and transferred to edible tissues.
29
Efficacy of Oxidizing Disinfectants at Inactivating Murine Norovirus on Ready-to-Eat Foods
Julie Jean
- 01 Aug 2016
TL;DR: In this article, the authors evaluated the efficacy of oxidizing disinfectants (sodium hypochlorite, chloride dioxide and peracetic acid) at inactivating noroviruses on fruits and vegetables, using a norovirus surrogate, which replicates in cell culture.
22
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