Open AccessJournal Article
Establishment and characterization of a human prostatic carcinoma cell line (PC-3).
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TL;DR: The establishment, characterization, and tumorigenicity of a new epithelial cell line (PC-3) from a human prostatic adenocarcinoma metastatic to bone is reported, which should be useful in investigating the biochemical changes in advanced prostatic cancer cells and in assessing their response to chemotherapeutic agents.
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Abstract: The establishment, characterization, and tumorigenicity of a new epithelial cell line (PC-3) from a human prostatic adenocarcinoma metastatic to bone is reported The cultured cells show anchorage-independent growth in both monolayers and in soft agar suspension and produce subcutaneous tumors in nude mice Culture of the transplanted tumor yielded a human cell line with characteristics identical to those used initially to produce the tumor PC-3 has a greatly reduced dependence upon serum for growth when compared to normal prostatic epithelial cells and does not respond to androgens, glucocorticoids, or epidermal or fibroblast gowth factors Karyotypic analysis by quinacrine banding revealed the cells to be completely aneuploid with a modal chromosome number in the hypotriploid range At least 10 distinctive marker chromosomes were identified The overall karyotype as well as the marker chromosomes are distinct from those of the HeLa cell Electron microscopic studies revealed many features common to neoplastic cells of epithelial origin including numerous microvilli, junctional complexes, abnormal nuclei and nucleoli, abnormal mitochondria, annulate lamellae, and lipoidal bodies Overall, the functional and morphologic characteristics of PC-3 are those of a poorly-differentiated adenocarcinoma These cells should be useful in investigating the biochemical changes in advanced prostatic cancer cells and in assessing their response to chemotherapeutic agents
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Citations
A serologic approach to the definition of human prostatic carcinoma antigens
TL;DR: Preliminary results indicate that the prostatic cancer patient humoral antibody response may be directed toward either altered histocompatibility complex antigens or toward antIGens physically associated with histocomplementary complex antigenes, which are relevant in the immunobiology of prostatic carcinoma.
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Real‐Time In Vivo Confocal Fluorescence Imaging of Prostate Cancer Bone‐Marrow Micrometastasis Development at the Cellular Level in Nude Mice
Shinji Miwa,Shinji Miwa,Makoto Toneri,Kentaro Igarashi,Shuya Yano,Hiroaki Kimura,Katsuhiro Hayashi,Norio Yamamoto,Hiroyuki Tsuchiya,Robert M. Hoffman +9 more
TL;DR: The ability to track bone marrow micrometastasis in real time at the cellular level provides a visual target for evaluating new therapeutics for this recalcitrant disease.
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Patent
NOVEL GnRH ANALOGUES WITH ANTITUMOUR EFFECTS AND PHARMACEUTICAL COMPOSITIONS THEREOF
Dezso Gaal,Adrienn Kalnay,Sándor Lovas,Imre Mezo,Richard F. Murphy,Istvan Palyi,Janos Seprodi,Henrietta Tanai,Istvan Teplan,Geza Toth,Gizella Turi,Zsolt Vadasz,B. Vincze +12 more
- 09 Aug 1995
TL;DR: In this article, the present invention provides pharmacologically active compounds of formula (I), the compounds are GnRH analogues according to the instant invention formulas and the compositions have an antitumor effect.
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Post-SELEX Optimization and Characterization of a Prostate Cancer Cell-Specific Aptamer for Diagnosis.
Esther Campos-Fernández,Letícia S Barcelos,Aline Gomes de Souza,Luiz Ricardo Goulart,Vivian Alonso-Goulart +4 more
- 13 Feb 2020
TL;DR: The RNA aptamer A4 was modified (mA4) by adding deoxyribonucleotides to its ends to remove the reactive 2' hydroxyl groups of RNA's sugar at the ends of the aptamer and to make it more stable to widespread RNase contamination in laboratories as mentioned in this paper.
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