Book Chapter10.1016/B978-0-08-016876-0.50102-X
Enzyme-linked immunosorbent assay, ELISA
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TL;DR: In this paper, the specificity of the DNP system was assessed by inhibition with hapten, and the reaction of immune serum against DNP with DNP-protein, adsorbed to the tubes, was completely inhibited by haptens in solution.
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About: This article is published in Immunochemistry. The article was published on 01 Jan 1971.
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References
Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation.
TL;DR: A postfixation in osmium tetroxide, even after long periods of storage, developed an image that—notable in the case of glutaraldehyde—was largely indistinguishable from that of tissues fixed under optimal conditions with osmia tetroxides alone.
•Journal Article
Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes.
Eva Engvall,Peter Perlmann +1 more
TL;DR: In the DNP system, the specificity of the reaction was assessed by inhibition with hapten, and the reaction of immune serum against DNP with DNP-protein, adsorbed to the tubes, was completely inhibited by haptens in solution.
3.5K
Specific carcinoembryonic antigens of the human digestive system
Phil Gold,Samuel O. Freedman +1 more
TL;DR: It was concluded that the carcinoembryonic antigens represent cellular constituents which are repressed during the course of differentiation of the normal digestive system epithelium and reappear in the corresponding malignant cells by a process of derepressive-dedifferentiation.
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Coupling of enzymes to proteins with glutaraldehyde. Use of the conjugates for the detection of antigens and antibodies.
TL;DR: Conjugation of peroxidase, glucose oxidase, tyrosinase and alkaline phosphatase to human immunoglobulin-G, human serum albumin, sheep antibody and rabbit antibody was carried out with glutaraldehyde to characterize the antibodies after immunoelectrophoresis.
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