Book Chapter10.1016/S0076-6879(80)70067-8
Enzyme immunoassay ELISA and EMIT.
1K
TL;DR: This chapter discusses the enzyme immunoassay ELISA and EMIT, where ELISA requires very little knowledge of enzyme technology, whereas enzymology is the key to success in EMIT.
read more
Abstract: Publisher Summary This chapter discusses the enzyme immunoassay ELISA and EMIT. Enzyme immunoassays can be classified into two fundamentally different types of assays: heterogeneous and homogeneous enzyme immunoassays (EIA). The heterogeneous EIA that include the enzyme-linked immunosorbent assay (ELISA) are based on the same principles as are used in radioimmunoassays (RIA). After incubation of antigen and antibodies, the antigen–antibody complexes formed are separated from free antigen and antibody by one of a number of different techniques and the activity in one or both of the fractions is determined. In the homogeneous enzyme immunoassay that includes enzyme multiplied immunoassay technique (EMIT), no such separation is necessary. The principle of EMIT is similar to the modified bacteriophage technique. Antigen-coupled enzyme shows a change in activity (infectivity) upon incubation with antibody. This change is inhibited when the binding of antibody to the antigen-coupled enzyme is prevented by the addition of free antigen. ELISA is generally applicable to the measurement of almost any antigen. In ELISA, the enzyme is a passive passenger through the actual immunoassay. In EMIT, the enzyme plays a key role throughout the assay process. ELISA requires very little knowledge of enzyme technology, whereas enzymology is the key to success in EMIT.
read more
Chat with Paper
AI Agents for this Paper
Find similar papers on Google Scholar, PubMed and Arxiv
Write a critical review of this paper
Analyze citations of this paper to find unaddressed research gaps
Citations
Monoclonal antibodies specific for apoproteins of lipophorins from the migratory locust
TL;DR: Monoclonal strains generated through cloning by limiting dilution from those hybridomas synthesizing antibodies specific for apolipophorins (apoLp)-I, -II, and -III of LDLp produced antibodies that bind to apoLp-III and native LDLp.
37
Evidence for the cell‐surface localization of antigens cross‐reacting with the “mitochondrial antibodies” of primary biliary cirrhosis
Iraj Ghadiminejad,Harold Baum +1 more
TL;DR: Studies with subfractions of Saccharomyces cerevisiae obtained by differential centrifugation showed only two primary biliary cirrhosis‐specific antigens, which were shown to have determinants cross‐reactive with their mammalian counterpart.
37
Anti-apoptotic genes, bag-1 and bcl-2, enabled hybridoma cells to survive under treatment for arresting cell cycle
Satoshi Terada,Katsuyuki Fukuoka,Tetsuo Fujita,Tomoaki Komatsu,Shinichi Takayama,John C. Reed,Eiji Suzuki +6 more
TL;DR: Genetic engineering of hybridoma cells for improving survival in the non-proliferating state will be useful for using nutrients in culture medium efficiently to produce antibody, since nutrients could be diverted from cell proliferation to antibody production in such non- Proliferates viable cell culture.
37
Characterization and purification of neutrophil ecto-phosphatidic acid phosphohydrolase.
Denis English,Margaret I. Martin,Kevin A. Harvey,Luke P. Akard,R D Allen,Theodore S. Widlanski,Joe G.N. Garcia,Rafat A. Siddiqui,Rafat A. Siddiqui +8 more
TL;DR: In the absence of detergent, short chain phosphatidic acids were hydrolysed most effectively by neutrophil plasma membrane ecto-PAPase; both saturated and unsaturated long chain phosph atidic acid were relatively resistant to hydrolysis.
36
Directing antigen specificity towards botulinum neurotoxin with combinatorial phage display libraries
Peter Emanuel,Thomas O'Brien,James Burans,Bibhuti R. DasGupta,James J. Valdes,Mohyee E. Eldefrawi +5 more
TL;DR: The production of recombinant immunoglobulin libraries in bacteria allows for a more controlled selection of antibody specificity and can be used in circumstances where hybridoma fusions are unable to isolate rare clones with the desired epitope specificity.
36
References
•Journal Article
Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes.
Eva Engvall,Peter Perlmann +1 more
TL;DR: In the DNP system, the specificity of the reaction was assessed by inhibition with hapten, and the reaction of immune serum against DNP with DNP-protein, adsorbed to the tubes, was completely inhibited by haptens in solution.
3.5K
Enzyme-linked immunosorbent assay, ELISA
TL;DR: In this paper, the specificity of the DNP system was assessed by inhibition with hapten, and the reaction of immune serum against DNP with DNP-protein, adsorbed to the tubes, was completely inhibited by haptens in solution.
3.3K
Peroxidase-labeled antibody. A new method of conjugation
Paul K. Nakane,Akira Kawaoi +1 more
TL;DR: A new method of conjugating horseradish peroxidase with proteins was developed by oxidizing the carbohydrate moiety with sodium periodate and bound to free amino groups of proteins unidirectionally at high efficiencies.
2.5K
Binding of soluble form of fibroblast surface protein, fibronectin, to collagen.
Eva Engvall,Erkki Ruoslahti +1 more
TL;DR: The findings suggest the possibility that Malignantly transformed fibroblasts lack surface fibronectin, which results in a lack of anchorage to the surrounding intercellular matrix, which could contribute to the malignant growth behavior.
2K