Journal Article10.1007/S10620-020-06389-X
Enteropathogenic Escherichia coli Infection Inhibits Intestinal Ascorbic Acid Uptake via Dysregulation of Its Transporter Expression
Christopher W. Heskett,Trevor Teafatiller,Carly Hennessey,Mélanie G. Gareau,Jonathan S. Marchant,Hamid M. Said,Hamid M. Said,Veedamali S. Subramanian,Veedamali S. Subramanian +8 more
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TL;DR: It is suggested that EPEC infection causes inhibition in AA uptake through a multifactorial dysregulation of SVCT1 and SVCT2 expression in intestinal epithelial cells.
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Abstract: Enteropathogenic Escherichia coli (EPEC) infection causes prolonged, watery diarrhea leading to morbidity and mortality Although EPEC infection impacts nutrient transporter function and expression in intestinal epithelial cells, the effects of EPEC infection on intestinal absorption of ascorbic acid (AA) have not yet been investigated To investigate the effect of EPEC infection on intestinal AA uptake process and expression of both AA transporters We used two experimental models: human-derived intestinal epithelial Caco-2 cells and mice 14C-AA uptake assay, Western blot, RT-qPCR, and promoter assay were performed EPEC (WT) as well as ΔespF and ΔespG/G2 mutant-infected Caco-2 cells showed markedly inhibited AA uptake, while other mutants (ΔescN, ΔespA, ΔespB, and ΔespD) did not affect AA uptake Infection also reduced protein and mRNA expression levels for both hSVCT1 and hSVCT2 EPEC-infected mice showed marked inhibitory effect on AA uptake and decreased protein and mRNA expression levels for both mSVCT1 and mSVCT2 in jejunum and colon MicroRNA regulators of SVCT1 and SVCT2 (miR103a, miR141, and miR200a) were upregulated significantly upon EPEC infection in both Caco-2 and mouse jejunum and colon In addition, expression of the accessory protein glyoxalate reductase/hydroxypyruvate reductase (GRHPR), which regulates SVCT1 function, was markedly decreased by EPEC infection in both models These findings suggest that EPEC infection causes inhibition in AA uptake through a multifactorial dysregulation of SVCT1 and SVCT2 expression in intestinal epithelial cells
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Citations
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John A. Chmiel,G. Stuivenberg,Kait F. Al,P. Akouris,Hassan Razvi,Jeremy P. Burton,Jennifer Bjazevic +6 more
TL;DR: In this paper , the role of the gut microbiota in kidney stone disease was investigated and the contribution of these vitamins to urolithiasis was reconsidered in the field, and investigation into previously overlooked vitamins (A, E and K) was expanded.
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Infection With Escherichia Coli Pathotypes Is Associated With Biomarkers of Gut Enteropathy and Nutritional Status Among Malnourished Children in Bangladesh
Md. Amran Gazi,Md. Ashraful Alam,Shah Mohammad Fahim,Barbie Zaman Wahid,Shaila S Khan,Md. Ohedul Islam,Mehedi Hasan,S. M. Tafsir Hasan,Subhasish Das,Mustafa Mahfuz,Rashidul Haque,Tahmeed Ahmed +11 more
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Histone deacetylase inhibitors regulate vitamin C transporter functional expression in intestinal epithelial cells.
TL;DR: In this paper, the authors found that SVCT1 expression and function were significantly decreased in intestinal epithelial cells by the histone deacetylase inhibitors (HDACi), valproic acid (VPA), and sodium butyrate (NaB).
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Effect of Lipopolysaccharide and TNFα on Neuronal Ascorbic Acid Uptake
Veedamali S. Subramanian,Trevor Teafatiller,Anshu Agrawal,Masashi Kitazawa,Jonathan S. Marchant +4 more
TL;DR: In this paper, the effect of LPS and TNFα on SVCT2 expression and function using in vitro and in vivo approaches was examined and the authors concluded that inflammatory signals suppress AA uptake by impairing SLC23A2 transcription through opposing regulation of Sp1 and NF-κB factors.
References
Analysis of relative gene expression data using real-time quantitative pcr and the 2(-delta delta c(t)) method
TL;DR: The 2-Delta Delta C(T) method as mentioned in this paper was proposed to analyze the relative changes in gene expression from real-time quantitative PCR experiments, and it has been shown to be useful in the analysis of realtime, quantitative PCR data.
168.2K
Vitamin C and Immune Function
Anitra C. Carr,Silvia Maggini +1 more
TL;DR: Prophylactic prevention of infection requires dietary vitamin C intakes that provide at least adequate, if not saturating plasma levels, and treatment of established infections requires significantly higher doses to compensate for the increased inflammatory response and metabolic demand.
1.6K
Molecular mechanisms of Escherichia coli pathogenicity
TL;DR: This Review focuses on the recent advances in the understanding of the different pathogenic mechanisms that are used by various E. coli pathovars and how they cause disease in humans.
Toward a new recommended dietary allowance for vitamin C based on antioxidant and health effects in humans
Anitra C. Carr,Balz Frei +1 more
TL;DR: The totality of the reviewed data suggests that an intake of 90-100 mg vitamin C/d is required for optimum reduction of chronic disease risk in nonsmoking men and women, suggesting a new RDA of 120 mg vitaminC/d.
947
Vitamin E and vitamin C treatment improves fibrosis in patients with nonalcoholic steatohepatitis
TL;DR: Vitamin E and vitamin C, in the doses used in this study, were well tolerated and were effective in improving fibrosis scores in NASH patients and a larger, multicenter, longer-term trial with vitamin E anditamin C seems to be warranted.
745