Journal Article10.1080/09205063.2012.759505
Engineering three-dimensional macroporous hydroxyethyl methacrylate-alginate-gelatin cryogel for growth and proliferation of lung epithelial cells
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TL;DR: The mechanical strength, stiffness, elastic measurements, in vivo compatibility, and in vitro lung cell proliferation show the potentiality of HAG materials for lung tissue engineering.
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Abstract: Three-dimensional (3D) growth of cell is of particular interest in the field of tissue engineering and regenerative medicine. Scaffolds used for this purpose are often tailor-made to mimic the microenvironment and the extracellular matrix of the tissue with defined role such as to provide appropriate structural, chemical, and mechanical support. The aim of the study was to design the macroporous matrix with potential in the field of tissue engineering especially for lung muscle regeneration. Blend of hydroxyethyl methacrylate-alginate-gelatin (HAG) cryogel scaffold was synthesized using cryogelation technique and this polymer material combination is being reported first time. The rheology study showed the elastic property of the material in wet state with no variation in storage modulus (G′), loss modulus (G″), and phase angle upon temperature variation. The microcomputer tomography (micro-CT) analysis confirmed the homogenous polymer structure with average pore diameter of 84 μm. Scaffold synthesized usi...
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Citations
Cryogels-versatile tools in bioseparation
Gizem Ertürk,Bo Mattiasson +1 more
TL;DR: High porosity, high mechanical and chemical stability make them appropriate carriers for immobilization of biomolecules and cells thereby making them attractive gel matrices for separation and purification of various molecules.
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Development of a Three-Dimensional Bioengineering Technology to Generate Lung Tissue for Personalized Disease Modeling
Dan C. Wilkinson,Jackelyn A. Alva-Ornelas,Jennifer M.S. Sucre,Preethi Vijayaraj,Abdo Durra,Wade Richardson,Steven J. Jonas,Manash K. Paul,Saravanan Karumbayaram,Bruce Dunn,Brigitte N. Gomperts +10 more
TL;DR: This method for the generation of self‐assembled human lung tissue and its potential for disease modeling and drug discovery for lung diseases characterized by progressive and irreversible scarring such as idiopathic pulmonary fibrosis (IPF).
Three-dimensional cryogels for biomedical applications.
TL;DR: The synthesis protocol and properties of cryogels, evaluation techniques as well as current in vitro and in vivo cryogel applications are summarized.
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Injectable Hyaluronic Acid-co-Gelatin Cryogels for Tissue-Engineering Applications.
TL;DR: It is suggested that hyaluronic acid-co-gelatin cryogels combined the favorable inherent properties of each biopolymer, providing a mechanically robust, cell-responsive, macroporous, and injectable platform for tissue-engineering applications.
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Modeling the lung: Design and development of tissue engineered macro- and micro-physiologic lung models for research use.
Joan E. Nichols,Jean A. Niles,Stephanie P. Vega,Lissenya B. Argueta,Adriene Eastaway,Joaquin Cortiella +5 more
TL;DR: The goal of engineering good 3D human models is the formation of physiologically functional respiratory tissue surrogates which can be used as pathogenesis models or in the case of 2D screening systems for drug therapy evaluation as well as human toxicity testing.
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