Journal Article10.1021/BC049858U
Engineering surfaces for bioconjugation: developing strategies and quantifying the extent of the reactions.
Virginie Gauvreau,Pascale Chevallier,Karine Vallières,Eric Petitclerc,René C.-Gaudreault,Gaétan Laroche +5 more
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TL;DR: Two-step and multistep reactions for modifying the surface of plasma-functionalized poly(tetrafluoroethylene) (PTFE) surfaces for subsequent conjugation of biologically relevant molecules and surface immobilization of fibronectin performed using either glutaric anhydride or sulfo-SMPB activators demonstrated the importance of selecting the appropriate conjugated strategy to retain the protein biological activity.
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About: This article is published in Bioconjugate Chemistry. The article was published on 20 Aug 2004. The article focuses on the topics: Bioconjugation & Fluoropolymer.
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Citations
Protein Immobilization Strategies for Protein Biochips
TL;DR: Current protein immobilization strategies, including physical, covalent, and bioaffinity immobilization for the fabrication of protein biochips, are described and particular consideration has been given to oriented immobilization, also referred to as site-specific immobilized, which is believed will improve homogeneous surface covering and accessibility of the active site.
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Chemical Strategies for Generating Protein Biochips
TL;DR: Different approaches using covalent and noncovalent chemistry are reviewed; particular emphasis is placed on the chemical specificity of protein attachment and on retention of protein function.
620
Immobilization of bio-macromolecules on self-assembled monolayers: methods and sensor applications
Debasis Samanta,Amitabha Sarkar +1 more
TL;DR: This critical review of the current developments in various techniques of immobilization of biomolecules, specifically biological macromolecules on surfaces through the modification of a functional self-assembled monolayer, highlights the utility of such immobilized biomolecule in the area of biosensing in nanoscale.
415
Orientation and characterization of immobilized antibodies for improved immunoassays (Review).
TL;DR: This review describes the most recent methods for oriented antibody immobilization and the characterization techniques employed for investigation of the antibody state.
340
Sulfobetaine-grafted poly(vinylidene fluoride) ultrafiltration membranes exhibit excellent antifouling property
TL;DR: In this article, the zwitterionic sulfobetaine methacrylate (SBMA) was grafted on the surface of poly(vinylidene fluoride) (PVDF) membrane via ozone surface activation and surface-initiated atom transfer radical polymerization (ATRP).
267
References
Immobilized RGD peptides on surface-grafted dextran promote biospecific cell attachment.
Stephen Massia,John Stark +1 more
TL;DR: With dextran-based surface coatings, it will be possible to develop well-defined surface modifications that promote specific cell interactions and perhaps better performance in long-term biomaterial implants.
228
X-Ray photoemission study of plasma modified polyethylene surfaces
TL;DR: In this article, X-ray photoelectron spectroscopy (XPS) was used to determine plasma induced chemical species on the surface of polyethylene (PE), and the degree of incorporation of new chemical species in the near surface region is approximately 20 at.
174
Plasma-treated PET surfaces improve the biocompatibility of human endothelial cells.
TL;DR: Polyethyleneterephthalate surfaces were treated by plasma processes with oxygen and ammonia and also in the presence of a gas mixture to verify the effect of functional groups grafting onto the endothelial cell growth, and cytocompatibility tests revealed an increase in cell growth with incubation time and the absence of well-spread and flattened cells.
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