Engineering microbial metabolic energy homeostasis for improved bioproduction

TL;DR: Engineered Escherichia coli biosynthesizes biodegradable poly(3-hydroxybutyrate-co-lactate) with varying lactate fractions by regulating electron transport chain activity through lepgt gene expression, achieving 10.6-41.8 mol% lactate with xylose as a carbon source.

TL;DR: In this article , a highly efficient micafungin-producing strain was constructed via systems metabolic engineering in C. empetri MEFC09, which was optimized by overexpressing the rate-limiting enzymes cytochrome P450 McfF and McfH.

TL;DR: Liquid-liquid equilibrium for ternary systems of water, methacrylic acid, and alkyl alcohols was investigated at 303.2 K and 101.3 kPa, with thermodynamic models (NRTL and UNIQUAC) correlating experimental data, indicating effective separation of methacrylic acid.

TL;DR: A new method to resolve the co Factor/redox imbalance problem is offered and the feasibility of the cofactor self-sufficient strategy for enhanced production of diverse chemicals is demonstrated, which improved the biocatalyst efficiency 3.8-fold.

TL;DR: Biophysical methods and crystallography are used to obtain a molecular-level description of the interactions between a carrier protein and an enzyme involved in bacterial sulfur oxidation, finding that similar principles underlie carrier protein interactions with other enzymes of the Sox pathway.

TL;DR: It is demonstrated that the energy-conserving glycerol utilization pathway GldA-DhaK can solve the energy problem during anaerobic fermentation of Glycerol to produce succinate.

TL;DR: The crystal structures of SoxAX from the photosynthetic bacterium Rhodovulum sulfidophilum have been solved, providing the first structural insights into the enzymatic oxidation of thiosulfate.