Engineering crops of the future: CRISPR approaches to develop climate-resilient and disease-resistant plants.
TL;DR: The latest developments in CRISPR technologies for engineering resistance to viruses, bacteria, fungi, and pests are discussed, highlighting current concerns and gaps in technology, as well as outstanding questions for future research.
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Abstract: To meet increasing global food demand, breeders and scientists aim to improve the yield and quality of major food crops. Plant diseases threaten food security and are expected to increase because of climate change. CRISPR genome-editing technology opens new opportunities to engineer disease resistance traits. With precise genome engineering and transgene-free applications, CRISPR is expected to resolve the major challenges to crop improvement. Here, we discuss the latest developments in CRISPR technologies for engineering resistance to viruses, bacteria, fungi, and pests. We conclude by highlighting current concerns and gaps in technology, as well as outstanding questions for future research.
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The CRISPR-Cas toolbox and gene editing technologies
TL;DR: A comprehensive review of CRISPR-Cas-related technologies can be found in this article , where the authors introduce and summarize the characteristics and applications of different types of CRispr-Cas tools.
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CRISPR–Cas-mediated chromosome engineering for crop improvement and synthetic biology
TL;DR: A review of the use of CRISPR-Cas technology to generate heritable and targeted chromosomal rearrangements for crop improvement and synthetic biology can be found in this paper, where the authors present a new way of manipulating genetic linkages, one in which the double-strand breaks (DSBs) are induced in somatic cells, enabling the formation of chromosomal inversions in the megabase range, by DSB repair via non-homologous end-joining.
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Advances in Crop Breeding Through Precision Genome Editing
Gauri Nerkar,Suman Devarumath,Madhavi V. Purankar,Atul Kumar,R. Valarmathi,Rachayya M. Devarumath,Chinnaswamy Appunu +6 more
TL;DR: This review includes an overview of the different breeding approaches for crop improvement; genome editing tools and their mechanism of action and application of the most widely used genome editing technology, CRISPR/Cas9, forcrop improvement especially for agronomic traits such as disease resistance, abiotic stress tolerance, herbicide tolerance, yield and quality improvement, reduction of anti-nutrients, and improved shelf life; and an update on the regulatory approval of the genome-edited crops.
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Host-mediated gene engineering and microbiome-based technology optimization for sustainable agriculture and environment
Nitika Thakur,Mohit Nigam,Neha Mann,Shivendra Gupta,Chaudhery Mustansar Hussain,Sudheesh K. Shukla,Anis Ali Shah,Ryan Casini,Hosam O. Elansary,Sher Aslam Khan +9 more
TL;DR: In this paper , a shift in the cultivation pattern from chemical to the novel, upgraded gene-assisted designed eco-friendly methodologies which can help in incorporating, exploring, and harnessing the right microbiome consortium and can further help in the progression of environmentally friendly microbiome technologies for agricultural safety and productivity.
Genome-editing in millets: current knowledge and future perspectives.
TL;DR: A review of genome editing studies in millets can be found in this article, where the authors discuss the future prospects of using genome editing to understand key traits of nutrient fortification and develop climate resilient crops in the future.
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