Engineering adipic acid metabolism in Pseudomonas putida.
Yannic S. Ackermann,Wing-Jin Li,Leonie Op de Hipt,Paul-Joachim Niehoff,William Casey,Tino Polen,Sebastian Köbbing,Hendrik Ballerstedt,Benedikt Wynands,Kevin E. O’Connor,Lars M. Blank,Nick Wierckx +11 more
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TL;DR: In this article, a reverse-engineered strain of Pseudomonas putida KT2440 was found to grow on adipate with a rate of 0.35 ± 0.01 h−1, reaching a final biomass yield of 0 27 − 0.27 − 1.00 gCDW gadipate−1.
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About: This article is published in Metabolic Engineering. The article was published on 07 May 2021. and is currently open access. The article focuses on the topics: Adipate & Pseudomonas putida.
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References
Pseudomonas putida as a functional chassis for industrial biocatalysis: From native biochemistry to trans-metabolism.
Pablo I. Nikel,Víctor de Lorenzo +1 more
TL;DR: A number of examples are presented for substantiating the worth of P. putida as one of the favorite workhorses for sustainable manufacturing of fine and bulk chemicals in the current times of the 4th Industrial Revolution.
416
Characterization and engineering of a two-enzyme system for plastics depolymerization.
Brandon C. Knott,Erika Erickson,Mark D. Allen,Japheth E. Gado,Rosie Graham,Fiona L. Kearns,Isabel Pardo,Ece Topuzlu,Jared J. Anderson,Harry P. Austin,Graham Dominick,Christopher W. Johnson,Nicholas A. Rorrer,Caralyn J. Szostkiewicz,Valérie Copié,Christina M. Payne,H. Lee Woodcock,Bryon S. Donohoe,Gregg T. Beckham,John McGeehan +19 more
TL;DR: The characterization of the MHETase enzyme and synergy of the two-enzyme PET depolymerization system may inform enzyme cocktail-based strategies for plastics upcycling and will inform future efforts in the biological deconstruction andUpcycling of mixed plastics.
396
Unique features revealed by the genome sequence of Acinetobacter sp. ADP1, a versatile and naturally transformation competent bacterium
Valérie Barbe,David Vallenet,Nuria Fonknechten,Annett Kreimeyer,Sophie Oztas,Laurent Labarre,Stéphane Cruveiller,Catherine Robert,Simone Duprat,Patrick Wincker,L. Nicholas Ornston,Jean Weissenbach,Philippe Marlière,Georges N. Cohen,Claudine Médigue +14 more
TL;DR: Genome analysis of the Acinetobacter ADP1 revealed genes for metabolic pathways involved in utilization of a large variety of compounds, supporting the view that the cycle itself is centrally geared to the catabolic capabilities of this exceptionally versatile organism.
Engineering multiple genomic deletions in Gram-negative bacteria: analysis of the multi-resistant antibiotic profile of Pseudomonas putida KT2440.
TL;DR: The directed edition of the P. putida chromosome shown here not only enhances the amenability of this bacterium to deep genomic engineering, but also validates the corresponding approach for similar handlings of a large variety of Gram-negative microorganisms.
381
Biotechnological domestication of pseudomonads using synthetic biology
TL;DR: This Review advocate the use of environmental Pseudomonas strains as model organisms that are pre-endowed with the metabolic, physiological and stress-endurance traits that are demanded by current and future synthetic biology and biotechnological needs.