Journal Article10.1016/J.JBIOSC.2011.05.017
Emulsion culture: a miniaturized library screening system based on micro-droplets in an emulsified medium.
Takaaki Kojima,Nobuhito Nagao,Daisuke Ando,Teruyo Ojima,Yasuaki Kawarasaki,Isao Kobayashi,Mitsutoshi Nakajima,Hideo Nakano +7 more
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TL;DR: A novel method of enrichment culture: "Emulsion Culture", i.e., segregated replication of clones in an emulsified culture medium, which allows a miniaturized, in-bulk screening of clones for screening for a secretory enzyme.
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About: This article is published in Journal of Bioscience and Bioengineering. The article was published on 01 Sep 2011.
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Citations
Signal peptide optimization tool for the secretion of recombinant protein from Saccharomyces cerevisiae.
Akihiro Mori,Shoichi Hara,Tomohiro Sugahara,Takaaki Kojima,Yugo Iwasaki,Yasuaki Kawarasaki,Takehiko Sahara,Satoru Ohgiya,Hideo Nakano +8 more
TL;DR: A novel method for the generation and rapid screening of a library of SP-target gene fusion constructs to identify the optimal SP for maximizing target protein secretion, and indicates that SPOT is a valuable method for optimizing the bioproduction of any target protein, and could be adapted to many host strains.
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Micro-compartmentalized cultivation of cyanobacteria for mutant screening using glass slides with highly water-repellent mark.
TL;DR: The droplet culture of cyanobacteria showed little toxicity using dodecane, and single cell encapsulation and culture can be a powerful tool in mutant selection.
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Evolutionary engineering to enhance starter culture performance in food fermentations
TL;DR: Recent developments of strain selection protocols and how computational approaches can assist experimental design of desired phenotypes are discussed and the analysis of evolved phenotypes and possibilities with complex consortia are highlighted.
High-throughput phenotyping of cell-to-cell interactions in gel microdroplet pico-cultures
Juliette Ohan,Juliette Ohan,Benjamin Pelle,Pulak Nath,J-H Huang,Blake T. Hovde,Momchilo Vuyisich,Dichosa Ae,Shawn R. Starkenburg +8 more
TL;DR: A validated high-throughput phenotyping pipeline is described that elucidates cell-to-cell interactions for millions of combinations of microorganisms and successfully isolated bacteria that improved algal growth.
References
Genome sequencing in microfabricated high-density picolitre reactors
Marcel Margulies,Michael Egholm,William E. Altman,Said Attiya,Joel S. Bader,Lisa A. Bemben,Jan Berka,Michael S. Braverman,Yi-Ju Chen,Zhoutao Chen,Scott Dewell,Lei Du,J. M. Fierro,Xavier V. Gomes,Brian C. Godwin,Wen He,Scott Edward Helgesen,Chun Heen Ho,Gerard P. Irzyk,Szilveszter C. Jando,Maria L. I. Alenquer,Thomas P. Jarvie,Kshama B. Jirage,Jong-Bum Kim,James R. Knight,Janna R. Lanza,John H. Leamon,Steven Lefkowitz,Ming Lei,Jing Li,Kenton Lohman,Hong Lu,Vinod Makhijani,Keith Mcdade,Michael P. McKenna,Eugene W. Myers,Elizabeth Nickerson,John Nobile,Ramona Plant,Bernard P. Puc,Michael T. Ronan,George T. Roth,Gary J. Sarkis,Jan Fredrik Simons,John Simpson,Maithreyan Srinivasan,Karrie R. Tartaro,Alexander Tomasz,Kari A. Vogt,Greg A. Volkmer,Shally H. Wang,Yong Wang,Michael P. Weiner,Pengguang Yu,Richard F. Begley,Jonathan M. Rothberg +55 more
TL;DR: A scalable, highly parallel sequencing system with raw throughput significantly greater than that of state-of-the-art capillary electrophoresis instruments with 96% coverage at 99.96% accuracy in one run of the machine is described.
Man-made cell-like compartments for molecular evolution
TL;DR: It is demonstrated the linkage of genotype to phenotype in man-made compartments using a model system and a selection for target-specific DNA methylation was based on the resistance of the product (methylated DNA) to restriction digestion.
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Ultrahigh-throughput screening in drop-based microfluidics for directed evolution
Jeremy J. Agresti,Eugene Antipov,Adam R. Abate,Keunho Ahn,Amy C. Rowat,Jean-Christophe Baret,Manuel Marquez,Alexander M. Klibanov,Andrew D. Griffiths,David A. Weitz +9 more
TL;DR: This work presents a general ultrahigh-throughput screening platform using drop-based microfluidics that overcomes limitations and revolutionizes both the scale and speed of screening.
•Journal Article
Correction for Ultrahigh-throughput screening in drop-based microfluidics for directed evolution
Jeremy J. Agresti,Eugene Antipov,Adam R. Abate,Keunho Ahn,Amy C. Rowat,Jean-Christophe Baret,Manuel Marquez,Alexander M. Klibanov,Andrew D. Griffiths,David A. Weitz +9 more
TL;DR: In this article, a drop-based microfluidics-based screening platform using aqueous drops dispersed in oil as picoliter-volume reaction vessels was proposed to identify new mutants of the enzyme horseradish peroxidase.
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Engineering of protein secretion in yeast: Strategies and impact on protein production
TL;DR: This mini-review focuses on recent strategies and their advantages for systematic engineering of yeast strains for effective protein secretion.
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