Electrospun Pectin-Polyhydroxybutyrate Nanofibers for Retinal Tissue Engineering
Siew Yin Chan,Siew Yin Chan,Benjamin Qi Yu Chan,Benjamin Qi Yu Chan,Zengping Liu,Bhav Harshad Parikh,Kangyi Zhang,Qianyu Lin,Qianyu Lin,Xinyi Su,Dan Kai,Wee Sim Choo,David J. Young,David J. Young,David J. Young,Xian Jun Loh,Xian Jun Loh +16 more
- 14 Dec 2017
- Vol. 2, Iss: 12, pp 8959-8968
TL;DR: It is proposed that nanofibers of pec-PHB have significant potential as retinal tissue engineering scaffold materials and were found to be noncytotoxic and biocompatible.
read more
Abstract: Natural polysaccharide pectin has for the first time been grafted with polyhydroxybutyrate (PHB) via ring-opening polymerization of β-butyrolactone. This copolymer, pectin-polyhydroxybutyrate (pec-PHB), was blended with PHB in various proportions and electrospun to produce nanofibers that exhibited uniform and bead-free nanostructures, suggesting the miscibility of PHB and pec-PHB. These nanofiber blends exhibited reduced fiber diameters from 499 to 336–426 nm and water contact angles from 123.8 to 88.2° on incorporation of pec-PHB. They also displayed 39–335% enhancement of elongation at break relative to pristine PHB nanofibers. pec-PHB nanofibers were found to be noncytotoxic and biocompatible. Human retinal pigmented epithelium (ARPE-19) cells were seeded onto pristine PHB and pec-PHB nanofibers as scaffold and showed good proliferation. Higher proportions of pec-PHB (pec-PHB10 and pec-PHB20) yielded higher densities of cells with similar characteristics to normal RPE cells. We propose, therefore, tha...
read more
Chat with Paper
AI Agents for this Paper
Find similar papers on Google Scholar, PubMed and Arxiv
Write a critical review of this paper
Analyze citations of this paper to find unaddressed research gaps
Citations
Biodegradable Polymers as the Pivotal Player in the Design of Tissue Engineering Scaffolds.
TL;DR: The interaction between implanted biodegradable biomaterials and the in vivo environment are discussed, including the properties and functional changes of the degradable scaffold, the local effect of degradation on the contiguous tissue and their evaluation using both in vitro and in vivo models.
211
Electrospun pectin/modified copper-based metal-organic framework (MOF) nanofibers as a drug delivery system.
Shahrzad Zirak Hassan Kiadeh,Azadeh Ghaee,Mehdi Farokhi,Jhamak Nourmohammadi,Addie Bahi,Frank Ko +5 more
TL;DR: In this article, a modified Cu-based MOF containing Folic acid was synthesized and incorporated in the suitable pectin electrospun nanofibers which not only improved the copper ions release behavior but also made the fiber mat stronger, antibacterial and induce angiogenesis, fibroblast migration, and proliferation due to loaded copper ions and folic acid.
102
Regenerative medicine and drug delivery: Progress via electrospun biomaterials.
TL;DR: This review attempts to critically and succinctly capture the vast number of developments reported in the literature over the past two decades of electrospinning as a versatile technique for producing nanofibers with specified physio-chemical characteristics suitable for diverse biomedical applications.
85
Recent progress in development and chemical modification of poly(hydroxybutyrate)-based blends for potential medical applications.
TL;DR: Various chemical modification approaches have been discussed and analyzed including hydroxylation, aminolysis, block copolymerization (via either grafting or blending) with suitable biopolymers, which may tune the mechanical and other desirable properties of resultant blends for value added biomedical applications particularly in drug delivery, tissue engineering and so on.
82
Use of biomaterials for sustained delivery of anti-VEGF to treat retinal diseases.
Ivan Seah,Xinxin Zhao,Qianyu Lin,Zengping Liu,Zengping Liu,Steven Zheng Zhe Su,Yew Sen Yuen,Walter Hunziker,Walter Hunziker,Walter Hunziker,Gopal Lingam,Xian Jun Loh,Xinyi Su +12 more
TL;DR: This review attempts to provide an overview of the sustained delivery platforms in their various phases of development for anti-VEGF agents to the posterior segment of the eye.
References
Alkylated pectin: Molecular characterization, conformational change and gel property
TL;DR: In this paper, the molecular characterization, conformational change and gel property of alkylated pectins (ALP) prepared by chemical covalent binding with different alkyl chains (C 6, C 12, or C 18 ) at different degrees of substitution (DS) were investigated.
46
Ocular tissue engineering: current and future directions.
TL;DR: Tissue engineering approaches for ocular tissues are available and have indeed come a long way, over the last decades; however more clinically relevant ocular tissue substitutes are needed.
42
Effect of sulfation on the physicochemical and biological properties of citrus pectins
TL;DR: In this paper, citrus pectins were subjected to chemical modification, specifically sulfation, and the antimicrobial and anticoagulant activities of the derivative were investigated, showing that the incorporation of sulfate groups into the pectin structure appeared to play an important role in improving its biological activities.
41
Poly(carbonate urethane)-Based Thermogels with Enhanced Drug Release Efficacy for Chemotherapeutic Applications.
Benjamin Qi Yu Chan,Benjamin Qi Yu Chan,Hongwei Cheng,Sing Shy Liow,Qingqing Dou,Yun-Long Wu,Xian Jun Loh,Xian Jun Loh,Zibiao Li +8 more
TL;DR: Studies conducted using nude mice models with hepatocellular carcinoma revealed that the Dox-loaded poly(PEG/PPG/PTHF carbonate urethane) thermogels showed excellent in vivo anti-tumour performance and effectively inhibited tumour growth in the tested model.
40
Effect of the film-processing conditions, relative humidity and ageing on wheat gluten films coated with electrospun polyhydryalkanoate
TL;DR: In this article, the influence of the processing conditions, the electrospun coating thickness and ageing on the water vapour and oxygen barrier properties and on the mechanical, morphological and optical properties of the materials have been evaluated.
40