Journal Article10.1016/J.SNB.2012.12.124
Electrochemical biosensing method for the detection of DNA methylation and assay of the methyltransferase activity
28
TL;DR: The inhibition investigation demonstrated that fisetin could inhibit the T.aqI MTase activity with the IC50 value of 280 μM, therefore, the screening of the inhibitors of MTase could be accomplished using the novel method.
read more
Abstract: In this article, we presented an electrochemical method for detection of DNA methylation and assay of DNA methyltransferase (MTase) activity using methylene blue (MB) as electrochemical indicator. After the DNA hybrid was methylated by T.aqI MTase, it could not be cleaved by HincII endonuclease. On the contrary, the double DNA without methylation could be cleaved, which would decrease the amount of intercalated MB. Thus, the DNA methylation status and MTase activity could be determinated based on the voltammetric signal change of MB. The methylation site could be found based on this method. The electrochemical signal of MB increased linearly with increasing T.aqI MTase concentration from 0.1 to 100 U/mL. Moreover, the inhibition investigation demonstrated that fisetin could inhibit the T.aqI MTase activity with the IC50 value of 280 μM. Therefore, the screening of the inhibitors of MTase could be accomplished using the novel method.
read more
Chat with Paper
AI Agents for this Paper
Find similar papers on Google Scholar, PubMed and Arxiv
Write a critical review of this paper
Analyze citations of this paper to find unaddressed research gaps
Citations
Nanotechnology-Enhanced No-Wash Biosensors for in Vitro Diagnostics of Cancer.
TL;DR: This review provides a comprehensive overview of various nanomaterial-enhanced no-wash biosensing technologies and focuses on the analysis of the underlying mechanism of these technologies applied for the early detection of cancer biomarkers ranging from small molecules to proteins, and even whole cancerous cells.
233
Electrochemical, electrochemiluminescent and photoelectrochemical bioanalysis of epigenetic modifiers: A comprehensive review
TL;DR: Electrochemical techniques for determining epigenetic biomarkers in last ten years are reviewed, in which the electrochemical techniques include electrochemistry, photoelectrochemistry and electrochemiluminescence, and the epigenetic modifiers contain DNA methylation, 5-methylcytosine and 7-methylguanine, DNA hydroxymethylation, DNA formylation, and RNA methylation.
111
Ultrasensitive homogeneous electrochemical strategy for DNA methyltransferase activity assay based on autonomous exonuclease III-assisted isothermal cycling signal amplification.
TL;DR: A simple and novel homogeneous electrochemical strategy for ultrasensitive DNA MTase activity assay has been successfully developed, which is based on methylation-triggered exonuclease (Exo) III-assisted autonomous isothermal cycling signal amplification.
81
DNA Methyltransferase Activity Assays: Advances and Challenges.
TL;DR: The progress in the development of DNA MTase activity assays is reviewed with an emphasis on assay mechanism and performance with some discussion on challenges and perspectives.
72
DNA Methylation Detection and Inhibitor Screening Based on the Discrimination of the Aggregation of Long and Short DNA on a Negatively Charged Indium Tin Oxide Microelectrode
TL;DR: The proposed system has the potential application to screen the drugs as inhibitors on the activity of methyltransferase in the clinic since it does not need complex operation procedures such as bisulfite treatment, PCR amplification, and electrode immobilization.
68
References
Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands
TL;DR: The use of MSP is demonstrated to identify promoter region hypermethylation changes associated with transcriptional inactivation in four important tumor suppressor genes (p16, p15, E-cadherin and von Hippel-Lindau) in human cancer.
6.1K
Gene Silencing in Cancer in Association with Promoter Hypermethylation
TL;DR: The mechanisms of gene silencing in cancer and clinical applications of this phenomenon are reviewed, especially tumor-suppressor genes.
3.6K
Cancer-epigenetics comes of age
Peter A. Jones,Peter W. Laird +1 more
TL;DR: Current mechanistic understanding of the role of DNA methylation in malignant transformation is reviewed, and it is suggested Knudson's two–hit hypothesis should be expanded to include epigenetic mechanisms of gene inactivation.
2.5K
DNA methylation in cancer: too much, but also too little.
TL;DR: The high frequency of cancer-linked DNA hypomethylation, the nature of the affected sequences, and the absence of associations with DNA hypermethylation are consistent with an independent role for DNA undermethylation in cancer formation or tumor progression.
1.6K
CpG island hypermethylation and tumor suppressor genes: a booming present, a brighter future.
TL;DR: Basic and translational studies will both be needed in the near future to fully understand the mechanisms, roles and uses of CpG island hypermethylation in human cancer.
1.3K