Efficient Cold Tolerance Evaluation of Four Species of Liliaceae Plants through Cell Death Measurement and Lethal Temperature Prediction
Woo Hyeong Yang,Seong Hyeon Yong,Dong-Jin Park,Sung-Hoon Ahn,Do Hyun Kim,Kwan-Been Park,Eon Ju Jin,Myung Suk Choi +7 more
TL;DR: In this article , the authors evaluated the cold tolerance of Liliaceae plants using visual inspection, electrolyte leakage, and the Evan's blue assay, and concluded that H. plantaginea had the highest cold tolerance, followed by H. longipes, S. scilloides, and H. fulva.
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Abstract: Although Liliaceae are valuable resource plants with medicinal and edible uses, techniques for evaluating their tolerance to various abiotic stresses are very limited. This study evaluated the cold tolerance using visual inspection, electrolyte leakage, and Evan’s blue assay. Visual inspection of the responses to different temperatures, using a temperature range of 4 to −12 °C, showed that Scilla scilloides was receive the least damage. However, electrolyte leakage tests showed slightly different results from visual inspection. The median lethal temperature (LT50) was expected to be between −4 and −8 °C. The LT50 was considered a measure of damage due to electrolyte leakage in plant cold tolerance evaluation. As a result of predicting the lethal temperature using the logistic regression equation, the average LT50 of the four plants was −9.0 °C. The species with the lowest LT50 was measured for Hosta plantagines (−11.14°C), whereas the highest LT50 was measured for Hemerocallis fulva (−7.14°C). As a result of the Evan’s blue assay, it was found that cell necrosis occurred when the plants were exposed to low temperatures. Visual observation showed that more than 50% of the three plants’ cells, except for H. plantaginea, were stained blue even at 8 °C. From this result, H. plantaginea was judged to have strong low-temperature tolerance. At −12 °C, more than 50% of the four Liliaceae plants were colored blue, and the LT50 value was expected to be below −12 °C. The reducing sugar content, an indicator of plant cold tolerance, was the highest in H. plantaginea, followed by S. scilloide and H. longipes. Combining the three methods, H. plantaginea had the highest cold tolerance, followed by H. longipes, S. scilloides, and H. fulva. The results of this study will be widely used in selecting cold-tolerant useful resource plants.
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Citations
Determining frost tolerance in <i>Lavandula</i>
E. Van Oost,Katrijn Van Laere,Peter Lootens,Bert De Rybel,Leen Leus +4 more
Unveiling Salt Tolerance Mechanisms and Hub Genes in Alfalfa (Medicago sativa L.) Through Transcriptomic and WGCNA Analysis
TL;DR: This study investigates salt tolerance mechanisms in alfalfa through transcriptomic and WGCNA analysis, identifying 5 hub genes related to salt stress and 2164 differentially expressed genes involved in oxidative stress response and metabolic pathways.
Enhancing Cold Tolerance Evaluation in Camellia sinensis and Camellia japonica Through Multimethod Analysis and Predictive Modeling
Woo-Hyeong Yang,Seong Hyeon Yong,Do-Hyun Kim,Kwan-Been Park,Ji Hyeon Lee,Seon A Kim,Jenna Jung,Myung Suk Choi,Woo-Hyeong Yang,Seong Hyeon Yong,Do-Hyun Kim,Kwan-Been Park,Ji Hyeon Lee,Seon A Kim,Jenna Jung,Myung Suk Choi +15 more
Abstract: Cold stress is a critical factor restricting the cultivation of subtropical evergreen species such as Camellia sinensis and C. japonica in temperate climates. This study aimed to develop an integrated framework for evaluating cold tolerance by combining visual assessment, electrolyte leakage (EL), Evans blue staining, and nonlinear regression modeling. All experiments were conducted with n = 3 samples per treatment, and statistical analyses were performed at a significance level of α = 0.05. Under freezing treatment at −6 °C, C. japonica exhibited faster and more severe damage, including leaf curling and vein darkening, compared to C. sinensis. Electrolyte leakage and cell death increased rapidly in C. japonica, and a sharp rise in cell death occurred in both species when EL exceeded 55%. Logistic regression of EL data estimated LT50 values of −10.96 °C for C. sinensis and −9.38 °C for C. japonica, while EL Temp50 values were −9.59 °C and −8.97 °C, respectively, indicating higher membrane stability in C. sinensis. The difference in LT50 between the two species was statistically significant (p < 0.05). Biochemical and heatmap analyses from 25 °C to −12 °C showed that C. sinensis maintained higher chlorophyll, antioxidant activity, and sugar levels, reflecting stronger cold tolerance. In contrast, C. japonica accumulated more proline and MDA, indicating higher stress sensitivity and membrane damage. This study presents a reproducible, quantitative framework for evaluating cold tolerance in Camellia species, offering valuable insights for breeding and expanding cultivation under climate change.
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