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Drosophila: A Laboratory Handbook
Michael Ashburner
- 01 Dec 1989
2K
TL;DR: This manual covers three approaches to the field: analysis of neural development, recording and imaging activities in the nervous system, and analysis of behavior.
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Abstract: Drosophila Neurobiology-Bing Zhang 2010 Based on Cold Spring Harbor Laboratory's long-running course, Drosophila Neurobiology: A Laboratory Manual offers detailed protocols and background material for researchers interested in using Drosophila as an experimental model for investigating the nervous system. This manual covers three approaches to the field: analysis of neural development, recording and imaging activities in the nervous system, and analysis of behavior. Techniques described include molecular, genetic, electrophysiological, imaging, behavioral and developmental methods.
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Citations
A novel hemocyte-specific membrane protein of Sarcophaga (flesh fly)
TL;DR: The immunoaffinity purification, cDNA cloning, and characterization of a prepupal hemocyte membrane protein of Sarcophaga (flesh fly) is reported, which is a novel type I transmembrane protein with 18 repeats of an epidermal growth factor-like domain in the predicted extracellular region.
17
Meiotic behaviour of a new complex X-Y-autosome translocation and amplified heterochromatin in Jumnos ruckeri (Saunders) (Coleoptera, Scarabaeidae, Cetoniinae).
TL;DR: As in the cases of X-autosome translocations in mammals, autosomal and gonosomal parts of the neo-sex chromosomes were insulated by heterochromatin, which may be a necessary condition to avoid deleterious position effects, whatever the mechanisms of gene dosage compensation.
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The cytoskeletal regulator zyxin is required for viability in Drosophila melanogaster.
Patricia J. Renfranz,Patricia J. Renfranz,Elizabeth Blankman,Mary C. Beckerle,Mary C. Beckerle +4 more
TL;DR: A zyx102 RNA‐interference transgenic line is generated that allows for the conditional knockdown of Zyx102, and if dissected from the pupal case, knockdown individuals appear morphologically normal, but remain inviable.
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Cranberry Interacts With Dietary Macronutrients to Promote Healthy Aging in Drosophila
Cecilia Wang,Jason Yolitz,Thomas Alberico,Mara Laslo,Yaning Sun,Charles T. Wheeler,Xiaoping Sun,Sige Zou +7 more
TL;DR: It is suggested that cranberry promotes healthy aging by increasing stress responsiveness and stresses the importance of considering diet composition in designing interventions for promoting healthy aging.
Heterochromatin-Associated Proteins HP1a and Piwi Collaborate to Maintain the Association of Achiasmate Homologs in Drosophila Oocytes
TL;DR: A speculative model is presented outlining how HP1a and Piwi could collaborate to keep achiasmate chromosomes associated in a homology-dependent manner and it is shown that Piwi is stably bound to numerous sites along the meiotic chromosomes, including centromere proximal regions.
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