DNA Joining method

TL;DR: In this paper, the authors describe novel polypeptides possessing part or all of the primary structural conformation and one or more of the biological properties of mammalian erythropoietin ("EPO") which are characterized in preferred forms by being the product of procaryotic or eucaryotic host expression of an exogenous DNA sequence.

TL;DR: In this article, the authors describe methods of preparing synthetic structural genes coding for the expression of mammalian polypeptides in microbial cloning systems, including plasmids suited for the transformation of bacterial hosts.

TL;DR: In this article, the analogs of human leukocyte interferon which predominantly include those amino acid residues which are common to all naturallyoccurring HIB subtype amino acid sequences and which include, at one or more of those positions, an amino acid which predominantly occurs at that position and in no event include any amino acid residue which is not extant in that position in at least one naturally-occurring subtype.