Distribution and phylogenetic comparisons of a novel mosquito flavivirus sequence present in Culex tarsalis Mosquitoes from western Canada with viruses isolated in California and Colorado.
Shaun Tyler,Bethany G. Bolling,Carol D. Blair,Aaron C. Brault,Kanti Pabbaraju,M. Veronica Armijos,David C. Clark,Charles H. Calisher,Michael A. Drebot +8 more
TL;DR: The ecology and geographic range of Calbertado virus warrants further study because it may potentially influence transmission of mosquito-borne flaviviruses, including important human pathogens such as West Nile and Saint Louis encephalitis viruses.
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Abstract: In a previous study, a new flavivirus genome sequence was identified in Culex tarsalis mosquitoes obtained in Alberta, Canada and was shown to be genetically related to but distinct from members of the insect-specific flaviviruses. Nonstructural protein 5-encoding sequences amplified from Cx. tarsalis pools from western Canada have shown a high similarity to genome sequences of novel flaviviruses isolated from mosquitoes in California and Colorado. Despite wide distribution of this virus, designated Calbertado virus, strains demonstrate a high degree of nonstructural protein 5 nucleotide (> 90%) and amino acid (> 97%) identity. The ecology and geographic range of Calbertado virus warrants further study because it may potentially influence transmission of mosquito-borne flaviviruses, including important human pathogens such as West Nile and Saint Louis encephalitis viruses.
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Citations
Insect-specific flaviviruses: a systematic review of their discovery, host range, mode of transmission, superinfection exclusion potential and genomic organization.
TL;DR: The discovery, host range, mode of transmission, superinfection exclusion ability, genomic organization and genomic organization of ISFs are reviewed.
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Transmission dynamics of an insect-specific flavivirus in a naturally infected Culex pipiens laboratory colony and effects of co-infection on vector competence for West Nile virus
TL;DR: It is found that vertical transmission is the primary mechanism for persistence of CxFV in Cx.
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A new insect-specific flavivirus from Northern Australia suppresses replication of West Nile Virus and Murray Valley Encephalitis Virus in co-infected mosquito cells
Jody Hobson-Peters,Alice Wei Yee Yam,Jennifer Lu,Yin Xiang Setoh,Fiona J. May,Nina Kurucz,Susan Walsh,Natalie A. Prow,Steven S. Davis,Richard Weir,Lorna Melville,Neville Hunt,Richard I. Webb,Bradley J. Blitvich,Peter I Whelan,Roy A. Hall +15 more
TL;DR: It is demonstrated that prior infection of cultured mosquito cells with PCV suppressed subsequent replication of the medically significant West Nile and Murray Valley encephalitis viruses by 10–43 fold, suggesting that superinfection exclusion can occur between ISFs and vertebrate-infecting flaviviruses despite their high level of genetic diversity.
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Development of one-step quantitative reverse transcription PCR for the rapid detection of flaviviruses
Pranav Patel,Olfert Landt,Marco Kaiser,Oumar Faye,Tanja Koppe,Ulrich Lass,Amadou A. Sall,Matthias Niedrig +7 more
TL;DR: The Pan-Flavi quantitative RT-PCR assay is rapid, broad-range flavivirus-specific and highly sensitive making it a valuable tool for rapid detection of flaviviruses in livestock samples, epidemiological studies or as useful complement to single flaviv virus-specific assays for clinical diagnosis.
Insect-Specific Flaviviruses from Culex Mosquitoes in Colorado, with Evidence of Vertical Transmission
TL;DR: Mosquitoes were collected in Colorado during 2006 and 2007 to examine spatial and seasonal patterns of risk for exposure to Culex vectors and West Nile virus, which led to the detection and subsequent isolation of two insect-specific flaviviruses: CuleX flavivirus (CxFV), which was first described from Japan, and a novel insect flaviv virus, designated Calbertado virus (CLBOV, which has also been detected in California and Canada.
References
MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) Software Version 4.0
TL;DR: Version 4 of MEGA software expands on the existing facilities for editing DNA sequence data from autosequencers, mining Web-databases, performing automatic and manual sequence alignment, analyzing sequence alignments to estimate evolutionary distances, inferring phylogenetic trees, and testing evolutionary hypotheses.
30.9K
Flaviviridae :T he Viruses and Their Replication
Brett D. Lindenbach Heinz-J,Charles M. Rice +1 more
- 01 Jan 2007
TL;DR: The present research attacked the Flavivirus infection through two mechanisms: Membrane Reorganization and the Compartmentalization and Assembly and Release of Particles from Flaviv virus-infected Cells and Host Resistance to Flaviviral Infection.
1.9K
Identification and Geographical Distribution of the Mosquitoes of North America, North of Mexico, 2nd Edition. By R. F. Darsie Jr. and R. A. Ward, pp. 416. University Press of Florida, USA, 2005. ISBN0 8130 2784 5. US$ 75.00.
TL;DR: A comprehensive and thorough work of scholarship is dedicated to the memory of the great American mosquito systematist and biologist, John N. Belkin this article, who added 31 species and revised the original keys.
469
Comparison of Flavivirus Universal Primer Pairs and Development of a Rapid, Highly Sensitive Heminested Reverse Transcription-PCR Assay for Detection of Flaviviruses Targeted to a Conserved Region of the NS5 Gene Sequences
Natale Scaramozzino,Jean-Marc Crance,Alain Jouan,Dominique André DeBriel,Françoise Stoll,Daniel Garin +5 more
TL;DR: It was confirmed that the sequenced amplified products in the NS5 region allowed predictability of flavivirus species by dendrogram, including the New York 99 West Nile strain.
424