Dissimilarity in protein chain elongation factor requirements between yeast and rat liver ribosomes.
L Skogerson,D Engelhardt +1 more
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TL;DR: The data suggest that the yeast EF-3 may be a loosely bound ribosomal protein which is not required for a specific step in the elongation cycle but is involved in the coordination of the partial reactions required for polymerization.
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About: This article is published in Journal of Biological Chemistry. The article was published on 25 Feb 1977. and is currently open access. The article focuses on the topics: Elongation factor & Yeast.
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Citations
Comparative analysis of ATPase of yeast elongation factor 3 and ATPase associated with Tetrahymena ribosomes
TL;DR: Comparative analysis of biochemical, immunological and functional properties of ATPase activity associated with the ribosomes isolated from the ciliated protozoan Tetrahymena pyriformis with that of yeast EF-3 ATPase indicates that these two activities are significantly different.
12
Eukaryotic polypeptide elongation system and its sensitivity to the inhibitory substances of plant origin.
TL;DR: It was noted that the components of elongation system can be inhibited, in separate steps, by the substances isolated from various sources of plant origin.
11
Reconstitution of yeast translation elongation and termination in vitro utilizing CrPV IRES-containing mRNA
TL;DR: It is found that a high eEF1A concentration relative to the ribosome concentration is critically required for efficient IRes-mediated translation initiation, to ensure its dominance over IRES-independent random internal translation initiation.
11
Antifungal drug development: the identification of new targets.
TL;DR: New antifungal targets have been identified through a combination of classical genetic, cytological and biochemical studies and are reviewed, as is the potential for applying recombinant DNA techniques as a means of confirming the role of the identified gene products in pathogenesis.
11
Eukaryotic Elongation Factor 3 Protects Saccharomyces cerevisiae Yeast from Oxidative Stress.
TL;DR: It is found that HEF3 is necessary to withstand oxidative stress conditions, suggesting that Hef3p is involved in the selective production of proteins that are necessary for defense against reactive oxygen species.
10
References
Requirement of an Escherichia coli 50 S ribosomal protein component for effective interaction of the ribosome with T and G factors and with guanosine triphosphate.
TL;DR: Escherichia coli ribosomes were made deficient in a 50 S ribosomal protein component by treatment with ethanol and NH4Cl and their ability to carry out a number of partial reactions involved in polypeptide synthesis was examined.
363
Determination of the number of proteins in liver ribosomes and ribosomal subunits by two-dimensional polyacrylamide gel electrophoresis.
Corinne C. Sherton,Ira G. Wool +1 more
TL;DR: The number of proteins in rat liver ribosome was determined by two-dimensional polyacrylamide gel electrophoresis and it is estimated that eukaryotic ribosomes contain between 68 and 72 different proteins.
252
Comparison of amino acid polymerization factors isolated from rat liver and rabbit reticulocytes
Luciano Felicetti,Fritz Lipmann +1 more
TL;DR: Using preliminary ammonium sulfate fractionation followed by calcium phosphate adsorption and elution, two complementary fractions, T 1 and T 2 , analogous to Schweet's TF-1 and TF-2, were obtained from liver as well as reticulocytes.
115
A ribosome-dependent GTPase from yeast distinct from elongation factor 2
TL;DR: Three proteins required for poly(U)-directed polyphenylalanine synthesis have been separated from yeast and it was found that one was a more active ribosome-dependent GTPase than elongation factor 2.
91