Journal Article10.1016/S0924-8579(98)00047-8
Discrepancies between mecA PCR and conventional tests used for detection of methicillin resistant Staphylococcus aureus.
74
TL;DR: A mecA PCR-based amplification was compared with the 1 microg oxacillin disk diffusion test and the Epsilometer test for detection of MICs and it was revealed that the reliable detection of MRSA cannot be solely based on detection ofmecA gene in S. aureus.
read more
About: This article is published in International Journal of Antimicrobial Agents. The article was published on 01 Jan 1999. The article focuses on the topics: SCCmec.
read more
Chat with Paper
AI Agents for this Paper
Find similar papers on Google Scholar, PubMed and Arxiv
Write a critical review of this paper
Analyze citations of this paper to find unaddressed research gaps
Citations
Laboratory Maintenance of Methicillin-Resistant Staphylococcus aureus (MRSA)
TL;DR: The four basic laboratory protocols presented in this unit describe how to culture S. aureus on liquid and solid media, how to identify S.aureus strains as methicillin resistant, and how to generate a freezer stock of S.Aureus for long-term storage.
Detection of methicillin/oxacillin resistance in staphylococci
TL;DR: The gold standard method for the detection of resistance mediated by mecA is PCR, which is most commonly used as a reference method at present, and some rapid and/or automated methods are also available, including latex agglutination techniques for the Detection of PBP2a.
Detecting methicillin resistance in Staphylococcus aureus: comparison of different phenotypic methods and the polymerase chain reaction
TL;DR: Performing the cefoxitin disk-diffusion method using a 30 | g disk could be a reliable and more accurate method to detect methicillin resistance in S. aureus strains in situations where mecA PCR cannot be performed.
Detection of methicillin resistance and slime factor production of Staphylococcus aureus in bovine mastitis
TL;DR: The optimized triplex PCR in this study was useful for rapid and reliable detection of methicillin resistant S. aureus and slime producing Staphylococcus aUREus in cases of bovine mastitis and only PCR targetting icaA and icaD may not sufficient to detect slime production.
References
National Committee for Clinical Laboratory Standards.
TL;DR: Many members of the Academy of Pediatrics seem to be generally unaware of the fact that the Academy has participated for ten years in a very interesting and valuable organization, the National Committee for Clinical Laboratory Standards (NCCLS).
14.1K
Methicillin resistance in staphylococci : Molecular and biochemical basis and clinical implications
TL;DR: Methicillin resistance in staphylococci is determined by mec, composed of 50 kb or more of DNA found only in methicillin-resistant strains, and methodologies based on the detection of mecA are the most accurate.
1.2K
Identification of methicillin-resistant strains of staphylococci by polymerase chain reaction.
TL;DR: The mecA gene, the structural gene of a low-affinity penicillin-binding protein (PBP 2'), was amplified by PCR and detected by agarose gel electrophoresis and conferred resistance on most of the bacteria.
1K
Methicillin-Resistant Staphylococcus aureus
TL;DR: In the early 1940s the introduction of benzylpenicillin (Penicillin G) temporarily solved the problem of staphylococcal infections, but the continued use of this agent caused the selection of resistant strains, which produced penicillinase (β-lactamase).
990