Journal Article10.1242/DEV.123.1.95
dino and mercedes, two genes regulating dorsal development in the zebrafish embryo
M. Hammerschmidt,Francisco Pelegri,Mary C. Mullins,D. A. Kane,F. J. M. Van Eeden,Michael Granato,Michael Brand,Makoto Furutani-Seiki,P. Haffter,Carl-Philipp Heisenberg,Y.-J. Jiang,Robert N. Kelsh,J. Odenthal,Rachel M. Warga,Christiane Nüsslein-Volhard +14 more
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TL;DR: The phenotypes suggests that dino and mercedes are required for the establishment of dorsal fates in both the marginal and the animal zone of the early gastrula embryo.
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Abstract: We describe two genes, dino and mercedes, which are required for the organization of the zebrafish body plan. In dino mutant embryos, the tail is enlarged at the expense of the head and the anterior region of the trunk. The altered expression patterns of various marker genes reveal that, with the exception of the dorsal most marginal zone, all regions of the early dino mutant embryo acquire more ventral fates. These alterations are already apparent before the onset of gastrulation. mercedes mutant embryos show a similar but weaker phenotype, suggesting a role in the same patterning processes. The phenotypes suggests that dino and mercedes are required for the establishment of dorsal fates in both the marginal and the animal zone of the early gastrula embryo. Their function in the patterning of the ventrolateral mesoderm and the induction of the neuroectoderm is similar to the function of the Spemann organizer in the amphibian embryo.
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References
Stages of embryonic development of the zebrafish.
TL;DR: A series of stages for development of the embryo of the zebrafish, Danio (Brachydanio) rerio is described, providing for flexibility and continued evolution of the staging series as the authors learn more about development in this species.
12K
•Book
Manipulating the mouse embryo: A laboratory manual
Brigid L.M. Hogan,Frank Costantini,Elizabeth Lacy +2 more
- 01 Jan 1986
TL;DR: Here are recorded the tech- niques for preparing, inserting and analysing DNA sequences, for retroviral infection of mice, for production and use of EC and EK cells as vehicles for engineered sequences and for nuclear transplantation - all against a background of the basic procedures required for pro- ducing and handling the em- bryos.
5.7K
•Book
Manipulating the Mouse Embryo: A Laboratory Manual
Richard R. Behringer,Marina Gertsenstein,Kristina Vintersten Nagy,Andras Nagy +3 more
- 15 Dec 2002
TL;DR: This chapter discusses developmental Genetics and Embryology of the Mouse, including production of Transgenic and Chimeric Mice, and techniques for Visualizing Gene Products, Cells, Tissues, and Organ Systems.
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