Differentiation of aspartic and isoaspartic acids using electron transfer dissociation
TL;DR: The lower resolution of the ion trap instrument makes detection of the aspartic acid residue’s diagnostic peak difficult because of interference with side-chain fragment ions from arginine residues, but the as partic acid residues are still clearly observed in the backbone cleavages and can be inferred from the absence of the isoas Partic acid diagnostic ions.
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About: This article is published in Journal of the American Society for Mass Spectrometry. The article was published on 01 Jan 2006. and is currently open access. The article focuses on the topics: Isoaspartic Acid & Electron-transfer dissociation.
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Citations
Middle-Down Proteomics Reveals Dense Sites of Methylation and Phosphorylation in Arginine-Rich RNA-Binding Proteins
TL;DR: It is suggested that the level of PTMs within Arg-rich domains may be among the highest in the proteome, and a possible unexplored regulator of RNA-binding protein interactions.
Identification of Sequence Similarities among Isomerization Hotspots in Crystallin Proteins
24 Feb 2017
TL;DR: The eye lens crystallins represent an ideal target for studying the effects of aging on protein structure and both collision-induced dissociation and radical-directed dissociation are needed for detection of these non-mass-shifting post-translational modifications.
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Chapter 16 Analysis of Deamidation in Proteins
TL;DR: A recently introduced mass spectrometric (MS) method uses electron capture dissociation (ECD) fragmentation patterns to distinguish aspartyl and isoaspartyl residues formed from deamidation of Asn residues, and suggests several methods to quantify them.
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Characterization of Synthetic Peptide Therapeutics Using Liquid Chromatography-Mass Spectrometry: Challenges, Solutions, Pitfalls, and Future Perspectives.
TL;DR: In this article, an overview of impurities in synthetic peptide therapeutics is provided in the context of how the knowledge from detailed characterization of the impurities using liquid chromatography-mass spectrometry (LC-MS) can be used to develop the manufacturing process and control strategy for synthetic peptides following the critical quality attribute (CQA)-driven and risk-based approach.
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Peptide and protein sequence analysis by electron transfer dissociation mass spectrometry
TL;DR: Peptide sequence analysis using a combination of gas-phase ion/ion chemistry and tandem mass spectrometry (MS/MS) is demonstrated and automated acquisition of high-quality, single-scan electron transfer dissociation MS/MS spectra of phosphopeptides separated by nanoflow HPLC is described.
Electron Capture Dissociation of Multiply Charged Protein Cations. A Nonergodic Process
TL;DR: In this article, it was shown that one or more charges on such protein cations can be neutralized with low energy electrons to cause specific cleavage of the amine bond to form c, z products, in contrast to the amide cleavage b, y products formed by collisionally activated dissociation (CAD),8 infrared multiphoton (IRMPD)9 and UV10 photodissociation, 70 eV electron impact excitation,11 and SID.
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Femtomole sequencing of proteins from polyacrylamide gels by nano-electrospray mass spectrometry
Matthias Wilm,Andrej Shevchenko,Tony Houthaeve,Stephen Breit,Lothar Schweigerer,Lothar Schweigerer,Theodore Fotsis,Matthias Mann +7 more
TL;DR: A simple and robust technique for the sequencing of proteins isolated by polyacrylamide gel electro-phoresis, using nano-electrospray3,4 tandem mass spectrometry5,6 and multiple-sequence stretches of up to 16 amino acids are obtained.
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Deamidation, isomerization, and racemization at asparaginyl and aspartyl residues in peptides. Succinimide-linked reactions that contribute to protein degradation.
Terrence L. Geiger,Steven Clarke +1 more
TL;DR: These studies indicate that both aspartic acid and asparagine residues may be hot spots for the nonenzymatic degradation of proteins, especially in cells such as erythrocytes and eye lens, where these macromolecules must function for periods of about 120 days and 80 years, respectively.
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Atmospheric Sampling Glow Discharge Ionization Source for the Determination of Trace Organic Compounds in Ambient Air
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