Journal Article10.1016/J.ACA.2013.05.065
Differential isotope dansylation labeling combined with liquid chromatography mass spectrometry for quantification of intact and N-terminal truncated proteins.
Yanan Tang,Liang Li +1 more
6
TL;DR: This work describes a rapid method for absolute quantification of protein mixtures containing intact and N-terminal truncated proteins and shows that dansyl labeled amino acids are stable in acidic conditions and can be quantified by liquid chromatography mass spectrometry with the use of isotope analog standards.
read more
About: This article is published in Analytica Chimica Acta. The article was published on 20 Aug 2013. The article focuses on the topics: Stable isotope labeling by amino acids in cell culture & Label-free quantification.
read more
Chat with Paper
AI Agents for this Paper
Find similar papers on Google Scholar, PubMed and Arxiv
Write a critical review of this paper
Analyze citations of this paper to find unaddressed research gaps
Citations
Development of Chemical Isotope Labeling LC-MS for Milk Metabolomics: Comprehensive and Quantitative Profiling of the Amine/Phenol Submetabolome.
Dorothea Mung,Liang Li +1 more
TL;DR: The high-coverage analysis of the milk metabolome using CIL LC-MS should be very useful in future research involving the study of the effects of these metabolites on human health and should also be useful in the dairy industry in areas such as improving milk production, developing new processing technologies, developing improved nutritional products, quality control, and milk product authentication.
42
PEP search in MyCompoundID: detection and identification of dipeptides and tripeptides using dimethyl labeling and hydrophilic interaction liquid chromatography tandem mass spectrometry.
TL;DR: A novel database-search strategy based on the use of MS/MS spectra of both unlabeled and dimethyl labeled peptides to identify and confirm amino acid sequences of di/tripeptides that are separated using hydrophilic interaction (HILIC) liquid chromatography (LC).
31
Dysregulated Lipid Synthesis by Oncogenic IDH1 Mutation Is a Targetable Synthetic Lethal Vulnerability
10 Nov 2022
TL;DR: In this article , the authors analyzed the metabolome of primary acute myeloid leukemia (AML) blasts and identified an mIDH1-specific reduction in fatty acids.
28
Direct infusion electrospray ionization-ion mobility-mass spectrometry for comparative profiling of fatty acids based on stable isotope labeling.
Jiapeng Leng,Qing Guan,Tuanqi Sun,Haoyang Wang,Jianlan Cui,Qinghao Liu,Zhixu Zhang,Manyu Zhang,Yinlong Guo +8 more
TL;DR: The integrated SIL technique and ESI-IM-MS are expected to serve as an alternative tool for high-throughput analysis of FAs in complex samples to minimized the matrix effect and variations in quantification and ensured accurate identification of non-targeted FAs by those typical features.
24
Isotope-labelling derivatisation: a broadly applicable approach to quantitation of algal toxins by isotope dilution LC-MS/MS
Daniel G. Beach,Christie Hollingdale,Christie Hollingdale,Michael A. Quilliam,Michael A. Quilliam +4 more
TL;DR: In this paper, isotope-labeling derivatisation is used for the analysis of algal biotoxins in complex biological and environmental samples to demonstrate the concept of isotope labeling derivatization for quantitation.
References
Screening for Unknown Mutations by a Bioluminescent Protein Truncation Test with Homogeneous Detection
TL;DR: A bioluminescent protein truncation test is reported, in which the detection of the nascent protein is performed directly in the expression mixture, within seconds, without the need for separation and purification.
6
Hydrolysis and amino acid composition analysis of proteins
TL;DR: The amino acid analysis technology needs further simplification and automation of the hydrolysis, chromatography and detection steps to withstand the pressure exerted by the other technologies.
Microwave fixation: Understanding the variables to achieve rapid reproducible results
TL;DR: Using microwave irradiation for rapid glutaraldehyde and osmium tetroxide fixation of tissues for electron microscopy yielded preservation equal or better than routine immersion fixation when a time interval, a cold spot, and an ice‐encased vial were used during microwave fixation.
Study of the dansylation reaction of amino acids, peptides and proteins
C. Gros,B. Labouesse +1 more
TL;DR: It is shown that a compromise for optima conditions of dansylation (pH and temperature) may be found, taking into account the rate of hydrolysis and the pK of the group to be dansyated.
Dansyl-peptides matrix-assisted laser desorption/ionization mass spectrometric (MALDI-MS) and tandem mass spectrometric (MS/MS) features improve the liquid chromatography/MALDI-MS/MS analysis of the proteome.
Giovanni Chiappetta,Sega Ndiaye,Emmanuelle Demey,Iman Haddad,Gennaro Marino,Angela Amoresano,Joëlle Vinh +6 more
TL;DR: Peptide dansylation proved to be a rapid and cheap method to improve the performance of liquid chromatography (LC)/MALDI-MS/MS analysis at the proteomic scale in terms of peptide detection and sequence coverage.