Journal Article10.1126/SCIENCE.2412294
Differential control of U1 small nuclear RNA expression during mouse development
107
TL;DR: The differential accumulation of embryonic U1 RNA's may influence the pattern of gene expression during early development and differentiation.
read more
Abstract: During normal mouse development the relative amounts of two types of U1 small nuclear RNA's (U1 RNA) change significantly. Fetal tissues have comparable levels of the two major types of mouse U1 RNA's, mU1a and mU1b, whereas most differentiated adult tissues contain only mU1a RNA's. Those adult tissues that also accumulate detectable amounts of embryonic (mU1b) RNA's (for example, testis, spleen, and thymus) contain a significant proportion of stem cells capable of further differentiation. Several strains of mice express minor sequence variants of U1 RNA's that are subject to the same developmental controls as the major types of adult and embryonic U1 RNA. The differential accumulation of embryonic U1 RNA's may influence the pattern of gene expression during early development and differentiation.
read more
Chat with Paper
AI Agents for this Paper
Find similar papers on Google Scholar, PubMed and Arxiv
Write a critical review of this paper
Analyze citations of this paper to find unaddressed research gaps
Citations
Human U2 small nuclear RNA genes contain an upstream enhancer.
TL;DR: By introducing marked human U2 genes into HeLa cells on SV40‐ and pUC13‐based vectors, it is found that transient expression of the marked U2 gene did not require the SV40 enhancer.
An abundant U6 snRNP found in germ cells and embryos of Xenopus laevis.
J Hamm,Iain W. Mattaj +1 more
TL;DR: The particle state of U snRNPs was analyzed in oocytes, eggs, embryos and testes from Xenopus laevis and two U6 snRNA elements have been identified that might be essential for nuclear migration in oocyte, but not for the formation of U4/6 sn RNPs in vitro.
89
Distribution of Tenr, an RNA-binding protein, in a lattice-like network within the spermatid nucleus in the mouse.
TL;DR: It is suggested that the Tenr protein may be involved in testis-specific nuclear posttranscriptional processes such as heterogeneous nuclear RNA (hnRNA) packaging, alternative splicing, or nuclear/cytoplasmic transport of mRNAs.
Regulation of alternative mRNA splicing: old players and new perspectives
TL;DR: Together this paints a highly dynamic picture of splicing regulation, where the final splice site choice is governed by the entire transcriptional environment of a gene and its cellular context.
87
Splicing of SV40 early pre-mRNA to large T and small t mRNAs utilizes different patterns of lariat branch sites.
TL;DR: Interestingly, the predominant branch sites utilized in splicing of large T RNA in vitro were found to differ in nuclear extracts from HeLa and human 293 cells, correlated with previously observed differences in the ratio of largeT to small t mRNAs produced in the two cell types.
86
References
The induction of differentiation in teratocarcinoma stem cells by retinoic acid
Sidney Strickland,Vijak Mahdavi +1 more
TL;DR: Using one embryonal carcinoma cell line, F9, it is found that retinoic acid at concentrations as low as 10(-9) M induces multiple phenotypic changes in the cultures in vitro, which are consistent with the proposition that retinosic acid induces differentiation of embryonal cancer cells into endoderm.
1.4K
Are snRNPs involved in splicing
TL;DR: Several lines of evidence are presented that suggest a direct involvement of snRNPs in the splicing of hnRNA molecules, including the observation that the nucleotide sequence at the 5′ end of U1 RNA exhibits extensive complementarity to those across splice junctions in hn RNA molecules.
1.2K
•Journal Article
Establishment and characterization of a cloned line of C3H mouse embryo cells sensitive to postconfluence inhibition of division.
TL;DR: No spontaneous transformation in vitro has been observed in the stock cultures transferred on a regular schedule and tests for tumorigenicity at all passages were negative.
1.1K
Antibodies to small nuclear RNAs complexed with proteins are produced by patients with systemic lupus erythematosus
Michael R. Lerner,Joan A. Steitz +1 more
TL;DR: It is argued that each of the six snRNAs exists in a separate small nuclear ribonucleoprotein (snRNP) complex with a total molecular weight of about 175,000.
1.1K