Genetic analysis of shoot regeneration from cotyledonary explants of Brassica napus was carried out by 7×7 diallel crosses using cultivars showing a different ability for regeneration. Both additive and dominant effects were significant, with the additive effect being more important than the dominant one. Dominant genes had a positive effect on shoot regeneration. Non-allelic interaction and average maternal effects were not detected, while specific the maternal one was significant. In the 5×5 sub-diallel table, the maternal effect became nonsignificant. The mean degree of dominance was 0.759. Broad- and narrow-sense heritabilities were 0.973 and 0.819, respectively, indicating that shoot regenera- tion ability can be easily transferred into economically important cultivars showing a low or an unresponsive ability.

Genetic analysis of shoot regeneration from cotyledonary explants in Brassica napus.

In this work,
nodal-disk segments (4-6 mm in diameter × 5-6 mm in length) were
obtained from established shoot culture, resulted from disinfected tomato
seedlings, and they were suitable to induce different organogenic pathway under
the influence of specific hormonal treatment. Application of BAP (1-2.5 mg/l)
alone or in combination of 0.5 mg/l NAA resulted in induction of shoot formation.
Somatic embryogenesis was rarely appeared (6%) when relatively low
concentration of BAP (1.5 mg/l) with low concentration of IAA (0.5 mg/l IAA)
was applied. Root induction was triggered when nodal explants or shoot cuttings
were cultured on MS medium with (1 mg/l IAA, IBA or NAA) or without auxins, but
the best result was obtained when 1 mg/l IAA was used. Application of 0.5 mg/l
NAA stimulated callus formation but the best result was obtained when the three
different phytohormoes were used (0.5 mg/l 2,4-D + 1 mg/l NAA + 0.5 mg/l BAP).
These results indicated that nodal segments, as described in this protocol, can
be used as alternative to other types of explants such as cotyledon, hypocotyl
and leaf explants.

/pdf/hormonal-requirements-trigger-different-organogenic-pathways-57u9sqac83.pdf

Hormonal Requirements Trigger Different Organogenic Pathways on Tomato Nodal Explants

Proteomics analysis and agrobacterium mediated transformation in tomato (lycopersicon esculentum mill.) cultivars

Diallel analysis of shoot-forming capacity among selected tomato [Lycopersicum esculentum]

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Diallel analysis of shoot-forming capacity among selected tomato [Lycopersicum esculentum]

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Citations

Genetic analysis of shoot regeneration from cotyledonary explants in Brassica napus.

Hormonal Requirements Trigger Different Organogenic Pathways on Tomato Nodal Explants

Proteomics analysis and agrobacterium mediated transformation in tomato (lycopersicon esculentum mill.) cultivars

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