Development of eukaryotic cell-free systems based on Chinese hamster ovary cells for the production of "difficult-to-express" proteins
Lena Thoring
- 01 Jan 2018
1
TL;DR: The water needs of this region have changed in recent years from being primarily for agricultural purposes to domestic and industrial uses currently and in the past have been for industrial and industrial reasons.
read more
Abstract: .................................................................................................................................... V List of abbreviations ................................................................................................................. VI
read more
Chat with Paper
AI Agents for this Paper
Find similar papers on Google Scholar, PubMed and Arxiv
Write a critical review of this paper
Analyze citations of this paper to find unaddressed research gaps
Citations
Erythropoietin Production from CHO Cells Grown in a Fluidized-Bed Bioreactor with Macroporous Beads
M-D. Wang,M. Yang,N. Huzel,Michael Butler +3 more
- 01 Jan 1999
TL;DR: The high volumetric yield of EPO attained in the Cytopilot bioreactor indicates its potential as a system for large-scale production.
3
References
Glycosyltransferases: structures, functions, and mechanisms.
TL;DR: The expected two-step double-displacement mechanism is rendered less likely by the lack of conserved architecture in the region where a catalytic nucleophile would be expected, and a mechanism involving a short-lived oxocarbenium ion intermediate now seems the most likely, with the leaving phosphate serving as the base.
1.9K
Raptor, a binding partner of target of rapamycin (TOR), mediates TOR action.
Kenta Hara,Yoshiko Maruki,Xiaomeng Long,Ken-ichi Yoshino,Noriko Oshiro,Sujuti Hidayat,Chiharu Tokunaga,Joseph Avruch,Kazuyoshi Yonezawa +8 more
TL;DR: Raptor is an essential scaffold for the mTOR-catalyzed phosphorylation of 4EBP1 and mediates TOR action in vivo and yields an array of phenotypes that closely resemble those produced by inactivation of Ce-TOR.
1.9K
X-ray structure of a voltage-dependent K+ channel
Youxing Jiang,Alice Lee,Jiayun Chen,Vanessa Ruta,Martine Cadene,Brian T. Chait,Roderick MacKinnon +6 more
TL;DR: The structure of KvAP, a voltage-dependent K+ channel from Aeropyrum pernix, is presented and a crystal structure of the full-length channel at a resolution of 3.2 Å is determined, which suggests that the voltage-sensor paddles move in response to membrane voltage changes, carrying their positive charge across the membrane.
1.8K
Discovery and development of sorafenib: a multikinase inhibitor for treating cancer
Scott Wilhelm,Christopher A. Carter,Mark Lynch,Timothy B. Lowinger,Jacques Dumas,Roger A. Smith,Brian Schwartz,Ronit Simantov,Susan Kelley +8 more
TL;DR: The discovery and continuing development of sorafenib is described, the first oral multikinase inhibitor that targets Raf and affects tumour signalling and the tumour vasculature.
1.7K
Adding New Chemistries to the Genetic Code
Chang C. Liu,Peter G. Schultz +1 more
TL;DR: The development of new orthogonal aminoacyl-tRNA synthetase/tRNA pairs has led to the addition of approximately 70 unnatural amino acids to the genetic codes of Escherichia coli, yeast, and mammalian cells, which provide new opportunities to generate proteins with enhanced or novel properties and probes of protein structure and function.