Development of a Peptide Reactivity Assay for Screening Contact Allergens

TL;DR: An optimal molar‐concentration is determined that achieves even higher predictive capacity than the original ADRA and a comparison of these results for 136 chemicals showed that ADRA testing with 4 mM solutions achieved a four percentage point improvement in accuracy over original AD RA and a two percentage points improvement over DPRA testing.

TL;DR: A new fluorescence-based method, 'HTS-DCYA assay', is proposed, which aims at the identification of reactive electrophiles based on their chemical reactivity toward a model fluorescent thiol, comparable to other in chemico methods such as Cys-DPRA.

TL;DR: Molecular mechanisms associated with irritant contact dermatitis are more difficult to investigate and demonstrate as they are very often based on reversible interactions with biological systems.

TL;DR: This work proposes a third category named ‘prehapten’ for nonreactive sensitizing molecules transformed into ‘haptens’ by simple chemical transformation and without requirement of a specific enzymatic system.

TL;DR: The Dichotomous Data Problem as discussed by the authors, the Two-Sample Dispersion Problem, and Other Two--Sample Problems are the most commonly used problems in survival analysis. But they do not consider the two-way layout.

TL;DR: High levels of cellular GSH levels in cultured cells in response to hyperoxia or electrophilic agents such as diethylmaleate is coupled with an increase in activity of the Xc- transport system for the amino acids cystine and glutamate.

TL;DR: High-performance liquid chromatography (HPLC) provides a rapid, sensitive, and reproducible means of separating and quantifying simultaneously a variety of sulfur-containing amino acids and related derivatives.