Development of a Peptide Reactivity Assay for Screening Contact Allergens
G. Frank Gerberick,Jeffrey D. Vassallo,Ruth E. Bailey,Joel G. Chaney,Morrall Stephen William,Jean-Pierre Lepoittevin +5 more
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TL;DR: The data presented show that measuring peptide reactivity has utility for screening chemicals for their skin sensitization potency and thus potential for reducing the authors' reliance on animal test methods.
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About: This article is published in Toxicological Sciences. The article was published on 01 Oct 2004. and is currently open access. The article focuses on the topics: Sensitization & Local lymph node assay.
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Citations
The use of Genomic Allergen Rapid Detection (GARD) assays to predict the respiratory and skin sensitising potential of e-liquids.
TL;DR: The initial results indicate that the GARD assays were able to differentiate and broadly classify e‐liquids based on their sensitisation potential, which are defined mixtures, and further studies need to be conducted to assess whether and how these assays could be used for the screening and toxicological assessment of e‐ liquids to support product development and commercialisation.
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1-Fluoro-2,4-dinitrobenzene and its derivatives act as secretagogues on rodent mast cells.
Yohei Manabe,Marie Yoshimura,Kazuma Sakamaki,Asuka Inoue,Aya Kakinoki,Satoshi Hokari,Mariko Sakanaka,Junken Aoki,Hiroyuki Miyachi,Kazuyuki Furuta,Satoshi Tanaka +10 more
TL;DR: In this paper, the potential of common experimental allergens to induce mast cell activation using murine bone marrow-derived cultured mast cells and rat peritoneal mast cells was investigated, and 1-chloro-2,4-dinitrobenzene (DNFB) was found to induce degranulation.
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A direct peptide reactivity assay using a high-throughput mass spectrometry screening platform for detection of skin sensitizers.
Zhengxi Wei,Yuhong Fang,Maya L. Gosztyla,Andrew J. Li,Wenwei Huang,Christopher A. LeClair,Anton Simeonov,Dingyin Tao,Menghang Xia +8 more
TL;DR: An automated 384-well plate-based RapidFire solid-phase extraction (SPE) system coupled with tandem mass spectrometry (MS/MS) DPRA was developed and validated in the presence of newly designed internal standard to improve assay throughput, sensitivity and accuracy.
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The activity of methacrylate esters in skin sensitisation test methods: A review.
TL;DR: The conclusions drawn are that – with this series of chemicals at least – there is a strong correlation between the results of animal tests and the in vitro assays considered, but not with either DEREK or TIMES‐SS.
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Proteomic analysis of haptenation by skin sensitisers: Diphencyprone and ethyl acrylate
Erika Parkinson,Maja Aleksic,Rachael Arthur,Sergio Regufe da Mota,Richard Cubberley,Paul Skipp +5 more
TL;DR: It is shown that haptenation in complex nucleophilic models is not random, but a specific, low level and reproducible event, and proteomic analysis extends the understanding of sensitiser reactivity beyond simple reactivity assays and offers a route to monitoring haptanation in living cells.
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