Development of a Peptide Reactivity Assay for Screening Contact Allergens

TL;DR: It was noted that a sufficient body of evidence now exists for conduct of methods other than the LLNA for carefully defined chemical classes, as well as for risk assessment, and progress towards methods which will deliver mainly hazard identification is being made.

TL;DR: Given the importance of regulatory applicability for 3R‐derived approaches to have a long‐lasting impact, this review examines the state of regulatory implementation and perspectives, respectively.

TL;DR: The current status of approaches used to measure the disposition of chemicals in skin compartments was reviewed, with particular emphasis on proposing recommendations on how best to use such information to reduce, refine and/or eliminate the need for animal testing, according to the Three Rs principle.

TL;DR: It is demonstrated that there is no unique PGE(2) inhibition mechanism: while the release of arachidonic acid from membrane phospholipids does not appear to be a target of modulation, COX-2 expression and/or COx-2 enzymatic activity are the major steps of prostaglandin synthesis that are inhibited by sensitizers.

TL;DR: The Dichotomous Data Problem as discussed by the authors, the Two-Sample Dispersion Problem, and Other Two--Sample Problems are the most commonly used problems in survival analysis. But they do not consider the two-way layout.

TL;DR: High levels of cellular GSH levels in cultured cells in response to hyperoxia or electrophilic agents such as diethylmaleate is coupled with an increase in activity of the Xc- transport system for the amino acids cystine and glutamate.

TL;DR: High-performance liquid chromatography (HPLC) provides a rapid, sensitive, and reproducible means of separating and quantifying simultaneously a variety of sulfur-containing amino acids and related derivatives.