Development and validation of RP-HPLC-PDA method for the quantification of eugenol in developed nanoemulsion gel and nanoparticles
TL;DR: In this paper, a photodiode array detector (PDA) was used for the quantification of eugenol in nanoemulsion gel and nanoparticles, and the method was validated in terms of linearity and range, accuracy, precision, specificity, robustness, detection limit, and quantitation limit.
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Abstract: Eugenol is a potent phytochemical, and a plethora of delivery systems for this bioactive agent is being developed. Reversed-phase high-pressure liquid chromatography equipped with photodiode array detector (RP-HPLC-PDA) method is very useful in the quantification of the phytochemicals. The RP-HPLC-PDA system with C18 reversed-phase column (250???4.6?mm, particle size 5??m) was used in this study. Acetonitrile and water in 1:1 (v/v) ratio was chosen as the mobile phase under a column temperature of 25?C. The detection wavelength was set at 280?nm with a flow rate of 1?mL/min. Method validation was performed according to the International Conference on Harmonization guidelines. HPLC method for the quantification of eugenol was successfully developed and validated. The method was validated in terms of linearity and range, accuracy, precision, specificity, robustness, detection limit, and quantitation limit. The developed RP-HPLC-PDA could be successfully employed for the quantification of eugenol in nanoemulsion gel and nanoparticles.
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Citations
Quantification and Brain Targeting of Eugenol-Loaded Surface Modified Nanoparticles Through Intranasal Route in the Treatment of Cerebral Ischemia.
TL;DR: Intranasal administration of developed CS-coated-EUG-loaded-PCL-NPs enhanced the drug bioavailability in rat brain and thus preparation of Eugenol-Nps may help treat cerebral ischemia effectively.
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Determination of Eugenol in Personal-Care Products by Dispersive Liquid-Liquid Microextraction Followed by Spectrophotometry Using p -Amino- N,N -dimethylaniline as a Derivatizing Agent
TL;DR: In this paper, two simple methods for the determination of eugenol were developed, one depends on the oxidative coupling of Eugenol with p-amino-N,N-dimethylaniline (PADA) in the presence of K 3 [Fe(CN) 6 ].
Eugenol-Encapsulated Nanocarriers for Microglial Polarisation: a Promising Therapeutic Application for Neuroprotection
TL;DR: In lipopolysaccharide-induced BV2 microglia cells, the two test concentrations of eugenol-loaded nanocarriers resulted in reduced nitrite concentration, indicating a switch from the pro-inflammatory to an anti-inflammatory state.
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Evaluating the anti-microbial effect of eugenol extracted from ocimum sanctum
TL;DR: From the results it can be concluded that the eugenol extracted from the powder and fresh leaves of tulsi has anti-microbial effect and the membrane composed of e Eugenol has the capability to retain the Eugenol.
References
Antioxidant and antibacterial activities of eugenol and carvacrol-grafted chitosan nanoparticles.
TL;DR: The grafted eugenol and carvacrol conferred antioxidant activity to the chitosan nanoparticles, and the essential oil component‐grafted chitotoxicity assays showed that the cytotoxicity of CHEU NPs and CHCA NPs were significant lower than those of the pure essential oils.
Eugenol: a natural compound with versatile pharmacological actions.
TL;DR: Eugenol possesses significant antioxidant, anti-inflammatory and cardiovascular properties, in addition to analgesic and local anesthetic activity, and the design of new drugs based on the pharmacological effects of eugenol could be beneficial.