Developing an Efficient protocol through Tissue culture Technique for Sugarcane Micropropagation

TL;DR: Experiments on shoot multiplication and rooting were laid out in a completely randomized design with factorial treatment arrangements to optimize in vitro protocol for mass propagation of two commercial sugarcane clones grown in Ethiopia through shoot tip culture.

TL;DR: The study verified that surface sterilization with Berekina 5% ingredients of chlorine for 25 min exposure time is optimal for sugarcane shoot tip decontamination and this treatment combination can replace sterilizationwith 0.1% mercury chloride for 10 min.

TL;DR: Two sugarcane varieties namely, ‘B41-227’ and ‘N14’ were tested for in vitro culture establishment response under five levels of BAP and kinetin and exhibited a better response than the other concentrations in percent initiated shoot tip explants, percent of dead or not responding cultures, number of shoots per explant and average shoot length.

TL;DR: In this article, the status, challenges and opportunities of in vitro technology for the sugar industry in Kenya are discussed and some problems have now been resolved to considerable extents which have been described in this review however, some constraints still require intensive research work to be resolved so that a safe and efficient exploitation of this technique can be ensured in sugarcane seed production programmes for enhanced yields and quality.