Journal Article10.1366/000370204322842931
Determination of Uronic Acids in Isolated Hemicelluloses from Kenaf Using Diffuse Reflectance Infrared Fourier Transform Spectroscopy (DRIFTS) and the Curve-Fitting Deconvolution Method
A. N. Batsoulis,M. K. Nacos,Christos S. Pappas,Petros A. Tarantilis,Thomas Mavromoustakos,M.G. Polissiou +5 more
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TL;DR: The proposed deconvolution method is simple, rapid, and nondestructive for the samples, and applicable in analysis of natural or synthetic mixtures and/or crude substances.
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Abstract: Hemicellulose samples were isolated from kenaf (Hibiscus cannabinus L.). Hemicellulosic fractions usually contain a variable percentage of uronic acids. The uronic acid content (expressed in polygalacturonic acid) of the isolated hemicelluloses was determined by diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS) and the curve-fitting deconvolution method. A linear relationship between uronic acids content and the sum of the peak areas at 1745, 1715, and 1600 cm(-1) was established with a high correlation coefficient (0.98). The deconvolution analysis using the curve-fitting method allowed the elimination of spectral interferences from other cell wall components. The above method was compared with an established spectrophotometric method and was found equivalent for accuracy and repeatability (t-test, F-test). This method is applicable in analysis of natural or synthetic mixtures and/or crude substances. The proposed method is simple, rapid, and nondestructive for the samples.
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Citations
Kenaf xylan – A source of biologically active acidic oligosaccharides
M. K. Nacos,Petros Katapodis,Christos S. Pappas,Dimitra Daferera,Petros A. Tarantilis,Paul Christakopoulos,Moschos G. Polissiou +6 more
TL;DR: Two xylose-rich hemicellulose fractions were obtained from kenaf wood through a series of sequential extractions which dissociated xylans from other cell wall components and used as substrates for the production of biologically active aldouronic acids.
153
Characterization of Bordetella pertussis growing as biofilm by chemical analysis and FT-IR spectroscopy
Alejandra Bosch,Diego O. Serra,Claudia Prieto,Juergen Schmitt,Dieter Naumann,Osvaldo Yantorno +5 more
TL;DR: The production of extracellular carbohydrates during biofilm growth was not associated with changes in the specific growth rate, growth phase, or oxygen limitation, and could represent an additional virulence factor that may help B. pertussis to evade host defenses.
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Surface characterization of stepwise oxidized spruce thermomechanical pulp samples by different analytical methods
TL;DR: In this paper, dye adsorption of various types of dye, namely Methylene Blue (MB), Crystal Violet (CV) and Astra Blue (AB) on the fiber surface was studied and compared to the kappa number and other fiber characteristics.
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Biotransformation of grape pomace from Vitis labrusca by Peniophora albobadia LPSC # 285 (Basidiomycota).
María Inés Troncozo,Cecilia Beatriz Fígoli,Mario Emilio Ernesto Franco,María Virginia Mirífico,Alejandra Bosch,Mario Rajchenberg,Pedro Alberto Balatti,Mario Carlos Nazareno Saparrat +7 more
TL;DR: It is concluded that Peniophora albobadia LPSC # 285 isolate is a promising fungus to transform grape pomace from Vitis labrusca under solid-state fermentation conditions.
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