Journal Article10.1021/JF801957H
Determination of ochratoxin a with a DNA aptamer.
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TL;DR: An aptamer that binds with high affinity and specificity to ochratoxin A (OTA), a mycotoxin that occurs in wheat and other foodstuffs, and a quantitative detection method for OTA based on the use of this aptamer are described.
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Abstract: This work describes the identification of an aptamer that binds with high affinity and specificity to ochratoxin A (OTA), a mycotoxin that occurs in wheat and other foodstuffs, and a quantitative detection method for OTA based on the use of this aptamer. Aptamers are single-stranded oligonucleotides selected in vitro to bind to molecular targets. The aptamer selected in this work exhibited a dissociation constant in the nanomolar range and did not bind compounds with structures similar to OTA such as N-acetylphenylalanine or warfarin. The aptamer bound with a 100-fold less affinity to ochratoxin B. The selected aptamers could be used for the determination of ppb quantities of OTA in naturally contaminated wheat samples. Further work is ongoing to broaden the application demonstrated here with the development of sensors, affinity columns, and other analytical systems for field and laboratory determination of this toxin in food and agricultural products.
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Citations
Recent Advances in Aptamer Discovery and Applications.
TL;DR: This review provides update on the recent advances in this rapidly progressing field of research with particular emphasis on generation of aptamers and their applications in biosensing, biotechnology and medicine.
521
Challenges and Opportunities for Small Molecule Aptamer Development
Maureen McKeague,Maria C. DeRosa +1 more
TL;DR: The technical challenges of aptamer development for small molecule targets, as well as the opportunities that exist for their application in biosensing and chemical biology are highlighted.
Ochratoxin A: 50 Years of Research
TL;DR: Careful analyses of the data show that OTA carcinogenic effects are due to combined direct and indirect mechanisms (e.g., genotoxicity, oxidative stress, epigenetic factors), which provides strong evidence that Ota carcinogenicity can also occur in humans.
409
Recent Advances in Optical Biosensors for Environmental Monitoring and Early Warning
Feng Long,An-Na Zhu,Hanchang Shi +2 more
TL;DR: Important advances in functional biorecognition materials (e.g., enzymes, aptamers, DNAzymes, antibodies and whole cells) that facilitate the increasing application of optical biosensors are reviewed.
315
Recent advances in recognition elements of food and environmental biosensors: a review
Bieke Van Dorst,Jaytry Mehta,Karen Bekaert,Elsa Rouah-Martin,Wim De Coen,Wim De Coen,Peter Dubruel,Ronny Blust,Johan Robbens +8 more
TL;DR: A sensitive monitoring of contaminants in food and environment, such as chemical compounds, toxins and pathogens, is essential to assess and avoid risks for both, human and environmental health, and there is a high need for sensitive, robust and cost-effective biosensors that make real time and in situ monitoring possible.
312
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