Journal Article10.1111/J.1471-4159.1978.TB06573.X
Determination of catecholamines in rat brain parts by reverse-phase ion-pair liquid chromatography
600
TL;DR: An improved method for the measurement of catecholamines in brain parts has been developed, based on reverse‐phase ion‐pair chromatography, which offers the advantages of high efficiency microparticulate liquid chromatography packings and the flexibility of ion‐ Pair chromatography.
read more
Abstract: —
An improved method for the measurement of catecholamines in brain parts has been developed, based on reverse-phase ion-pair chromatography. The new method offers the advantages of high efficiency microparticulate liquid chromatography packings and the flexibility of ion-pair chromatography. By this approach norepinephrine and dopamine (DA) have been measured in the hypothalamus and corpus striatum of the rat brain during various stages of development (15, 21, 30 days). Data are reported on the basis of the whole part and per weight of tissue. For the adult animals, the following concentrations (ng/g wet tissue) were observed for the hypothalamus: NE = 2261 ± 274, DA = 440 ± 103, and for the corpus striatum: DA = 11,888 ± 1840. The overall precision of the method was ±5.6% relative s.d. The absolute recovery was 60 ± 5% relative s.d. and was constant over the range of 1 ng to 1 μg of dopamine or norepinephrine per tissue sample. The relative retention behavior of 18 neurologically important catechol derivatives is reported for reverse-phase chromatography with octyl sulfate as the stationary phase modifier.
read more
Chat with Paper
AI Agents for this Paper
Find similar papers on Google Scholar, PubMed and Arxiv
Write a critical review of this paper
Analyze citations of this paper to find unaddressed research gaps
Citations
Regulation of noradrenergic function by inflammatory cytokines and depolarization
TL;DR: CT‐1 and depolarization regulate expression of noradrenergic properties in an opposing manner, and the combined treatment results in elevated cellular catecholamines and decreased NE uptake relative to control cells.
74
Chronoamperometry in brain slices: quantitative evaluations of in vivo electrochemistry.
TL;DR: Study in brain slices combined with similar data from intact animals have provided important new information on the nature of the electrochemical studies, and inhibiting monoamine oxidase activity with pargyline results in the second most important contributor in caudate extracellular fluid being 3,4-dihydroxyphenylacetic acid.
74
Effects of 5-HT and 8-OH-DPAT on forebrain monoamine synthesis after local application into the median and dorsal raphe nuclei of the rat.
TL;DR: Dissociation of the effects of 5- HT and 8-OH-DPAT on forebrain 5-HT synthesis after local application into the dorsal or the median raphe strongly supports the contention of heterogeniety in the brain 5-ht receptor population in terms of receptor subtypes and/or receptor regulation.
73
The neurotoxin MPTP does not reproduce in the rhesus monkey the interregional pattern of striatal dopamine loss typical of human idiopathic Parkinson's disease.
TL;DR: In rhesus monkeys MPTP failed to reproduce the interregional caudate-putamen gradient characteristic of idiopathic PD, and the DA pattern produced by MPTP was similar to the DA loss in caudates and putamen observed in patients with postencephalitic parkinsonism.
72
References
Catecholamines in fetal and newborn rat brain.
Joseph T. Coyle,D. Henry +1 more
TL;DR: An investigation of the effects of reserpine, pheniprazine, α‐methyl‐para‐tyrosine, diethyldithiocarbamate and l ‐DOPA on the levels of dopamine and norepinephrine in the brains of the 18‐day gestational fetus indicated that the levelsof these neurotransmitters are under controls similar to those known to occur in the brain of the adult rat.
793
Liquid chromatographic analysis of catecholamines routine assay for regional brain mapping
TL;DR: A thoroughly tested and highly reliable catecholamine assay is described which routinely determines 20–100 picograms of norepinephrine and dopamine in small punches of brain tissue weighing 0.50 to 50 mg.
733
Estimation of catecholamines in urine by high performance liquid chromatography with electrochemical detection.
TL;DR: A new method for simultaneous assay of 3,4-dihydroxyphenylalanine, dopamine, norepinephrine, and epinephrine in urine is described, suitable for routine clinical measurement of urinary catecholamines.
159
Recent developments in the clinical assessment of the metabolism of aromatics by high-performance, reversed-phase chromatography with amperometric detection.
TL;DR: High-performance liquid chromatography with electrochemical detection has developed into a tool with excellent capability to monitor picomole amounts of individual metabolites in both tissue and body-fluid specimens and has provided the foundation for several clinical assays now in routine use.
65