Detection rates of bacteria in chronic otitis media with effusion in children.
Chul Won Park,Jang Hee Han,Jin Hyeok Jeong,Seok Hyun Cho,Mi Jung Kang,Kyung Tae,Seung Hwan Lee +6 more
TL;DR: It is concluded that PCR is a more sensitive method for the detection of bacteria in middle ear effusion than ordinary culture, and acute Otitis media is a major contributor to the pathogenesis of otitis media with effusion.
read more
Abstract: This study was performed to investigate polymerase chain reaction-based detection of bacterial DNA in middle ear fluid and assess the correlation between the PCR-positive rate with several factors associated with middle ear effusion. The purpose was to gain a further understanding of bacterial infection as a major cause of otitis media with effusion. Of the 278 specimens of middle ear fluid, 39 (14%) tested positive by ordinary culture. The overall detection rate of bacterial DNA using the PCR method was 36.7% for middle ear effusion, and bacterial DNA detection rates of Hemophilus influenzae, Streptococcus pneumoniae, Moraxella catarrhalis in the middle ear effusion were 29.1%, 4.7% and 10.8%, respectively. The bacterial DNA detection rate was higher in ears with a history of acute otitis media than those without the history. High detection rates were observed in patients younger than 48 months who have had a higher tendency to present with acute otitis media. We concluded that PCR is a more sensitive method for the detection of bacteria in middle ear effusion than ordinary culture, and acute otitis media is a major contributor to the pathogenesis of otitis media with effusion.
read more
Chat with Paper
AI Agents for this Paper
Find similar papers on Google Scholar, PubMed and Arxiv
Write a critical review of this paper
Analyze citations of this paper to find unaddressed research gaps
Citations
Predominant Bacteria Detected from the Middle Ear Fluid of Children Experiencing Otitis Media: A Systematic Review
TL;DR: Ongoing monitoring with appropriate detection methods for OM pathogens can support development of improved vaccines to provide protection from the complex combination of otopathogens within the middle ear, ultimately aiming to reduce the risk of chronic and recurrent OM in vulnerable populations.
Multi-species bacterial biofilm and intracellular infection in otitis media
Ruth B. Thornton,Ruth B. Thornton,Paul Rigby,Selma P. Wiertsema,Selma P. Wiertsema,Pierre Filion,Jennifer Langlands,Harvey Coates,Harvey Coates,Shyan Vijayasekaran,Shyan Vijayasekaran,Anthony D. Keil,Peter Richmond,Peter Richmond +13 more
TL;DR: Using FISH and CLSM, bacterial biofilm and intracellular infection with known otopathogens are demonstrated on/in the middle ear mucosa of children with COME and/or rAOM.
PCR-based detection of bacterial DNA after antimicrobial treatment is indicative of persistent, viable bacteria in the chinchilla model of otitis media
J.C. Post,J. J. Aul,G. J. White,R. M. Wadowsky,T. Zaroval,R. Tabari,B. Kerber,W. J. Doyle,G. D. Ehrlich +8 more
- 01 Jan 1996
TL;DR: Findings indicate that purified DNA and DNA from intact but nonviable bacteria do not persist in the middle-ear cleft in the presence of an effusion, even following high copy inoculation.
110
Subinhibitory Concentrations of Azithromycin Decrease Nontypeable Haemophilus influenzae Biofilm Formation and Diminish Established Biofilms
TL;DR: Data show that subinhibitory concentrations of azithromycin have antibiofilm properties, provide mechanistic insights, and supply an additional rationale for the use of azathromycin in chronic biofilm infections involving H. influenzae.
97
Multiple combination antibiotic susceptibility testing of nontypeable Haemophilus influenzae biofilms.
Robert Slinger,Francis K.L. Chan,Wendy Ferris,Sau-Wai Yeung,Melissa St. Denis,Isabelle Gaboury,Shawn D. Aaron +6 more
TL;DR: Biofilm testing reveals differences in effectiveness among antibiotics not apparent from conventional susceptibility testing, and suggests novel antibiotic regimens that could be studied for treatment of OME.
87
References
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
Randall Keichi Saiki,David H. Gelfand,Susanne Stoffel,Stephen J. Scharf,Russell Higuchi,Glenn Thomas Horn,Kary B. Mullis,Henry A. Erlich +7 more
TL;DR: A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction, which significantly improves the specificity, yield, sensitivity, and length of products that can be amplified.
Molecular Analysis of Bacterial Pathogens in Otitis Media With Effusion
J. C. Post,Preston Ra,Aul Jj,Larkins-Pettigrew M,J. Rydquist-White,Anderson Kw,Robert M. Wadowsky,Reagan Dr,Walker Es,Lawrence A. Kingsley,Magit Ae,Garth D. Ehrlich +11 more
TL;DR: The PCR-based assay systems can detect the presence of bacterial DNA in a significant percentage of culturally sterile middle ear effusions, and the large number of bacterial genomic equivalents present in the ears is suggestive of an active process.
311
Evidence of bacterial metabolic activity in culture-negative otitis media with effusion.
Mark G. Rayner,Yingze Zhang,Michael C. Gorry,Yiping Chen,J. Christopher Post,Garth D. Ehrlich +5 more
TL;DR: The RT-PCR-based assay system can detect the presence of bacterial mRNA in a significant percentage of culturally sterile middle ear effusions, establishing the presenceof viable, metabolically active, intact organisms in some culture-negative OME.
304
PCR-based detection of bacterial DNA after antimicrobial treatment is indicative of persistent, viable bacteria in the chinchilla model of otitis media
J. Christopher Post,Jerome J. Aul,Gregory J. White,Robert M. Wadowsky,Thomas Zavoral,Reza Tabari,Brenda Kerber,William J. Doyle,Garth D. Ehrlich +8 more
TL;DR: In this paper, the authors used a single-point longitudinal study to determine whether the presence of viable bacteria or residual DNA in the middle-ear cleft of chinchillas was determined.
121
Comparison of PCR assay with bacterial culture for detecting Streptococcus pneumoniae in middle ear fluid of children with acute otitis media.
TL;DR: P pneumolysin PCR is a sensitive and specific new method to study pneumococcal involvement in MEF samples of children with AOM by comparing a newly developed pneumitiscal PCR for Streptococcus pneumoniae to bacterial culture with 180 middle ear fluid samples of 125 children with 125 episodes of AOM.
111
Related Papers (5)
Luanne Hall-Stoodley,Fen Z. Hu,Fen Z. Hu,Armin Gieseke,Armin Gieseke,Laura Nistico,Duc Nguyen,Jay Hayes,Michael L. Forbes,David P. Greenberg,Bethany Dice,Amy Burrows,P. Ashley Wackym,Paul Stoodley,Paul Stoodley,J. Christopher Post,J. Christopher Post,Garth D. Ehrlich,Garth D. Ehrlich,Joseph E. Kerschner +19 more