Detection of Salmonella spp. by a loop‐mediated isothermal amplification (LAMP) method targeting bcfD gene
TL;DR: The experiments show that the LAMP method developed is a rapid, sensitive, specific and practical method for Salmonella detection and can potentially serve as new on‐site diagnostics in the food and agricultural industries.
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Abstract: UNLABELLED In this study, we developed and validated a loop-mediated isothermal amplification (LAMP) assay for Salmonella detection targeting bcfD gene, a conserved fimbrial operon gene existing in Salmonella. The Salmonella LAMP assay we developed successfully amplified 44 Salmonella strains (14 standard strains and 30 clinical isolates), but none of 9 non-Salmonella standard strains (Proteus mirabilis, Listeria monocytogenes, Escherichia coli, Pseudomonas aeruginosa, Shigella flexneri, Shigella sonnei, Klebsiella pneumoniae, Campylobacter jejuni and Vibrio parahemolyticus). The detection limit was 5 CFU of Salmonella pure culture or 200 CFU of artificially spiked faeces per reaction system (equivalent to 5000 CFU g(-1) of faeces), and this method could directly detect Salmonella in chicken faeces free of pre-enrichment in a reaction time of 25 min. Our experiments show that the LAMP method we developed is a rapid, sensitive, specific and practical method for Salmonella detection. The Salmonella LAMP assay can potentially serve as new on-site diagnostics in the food and agricultural industries. SIGNIFICANCE AND IMPACT OF THE STUDY A loop-mediated isothermal amplification (LAMP) assay was established to detect Salmonella targeting bcfD gene, a conserved fimbrial operon gene. The detection limit was 5 CFU of Salmonella pure culture or 200 CFU of artificially spiked faeces per reaction system (equivalent to 5000 CFU g(-1) of faeces), and this method could directly detect Salmonella in chicken faeces free of pre-enrichment in a reaction time of 25 min. The Salmonella LAMP assay is a rapid, sensitive, specific and practical method for Salmonella detection and can potentially serve as new on-site diagnostics in the food and agricultural industries.
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Citations
Loop-Mediated Isothermal Amplification for Salmonella Detection in Food and Feed: Current Applications and Future Directions.
TL;DR: This review provides an overview of international efforts in the past decade on the development and application of Salmonella LAMP assays in a wide array of food and feed matrices.
Recent advances in loop-mediated isothermal amplification (LAMP) for rapid and efficient detection of pathogens
TL;DR: Loop-mediated isothermal amplification (LAMP) has been demonstrated to be a reliable and robust method for detection and identification of viral and microbial pathogens for both laboratory and on-field settings as mentioned in this paper .
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Development of a novel loop-mediated isothermal amplification (LAMP) assay for the detection of Salmonella ser. Enteritidis from egg products
Lijun Hu,Lijun Hu,Li Ma,Shimin Zheng,Xiaohua He,Thomas S. Hammack,Eric W. Brown,Guodong Zhang +7 more
TL;DR: In this paper, a loop-mediated isothermal amplification (LAMP) was used for the detection of Salmonella ser. Enteritidis, encoding fimbrial biosynthesis protein.
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Virulence and Antimicrobial Resistance Profiles of Salmonella enterica Serovars Isolated from Chicken at Wet Markets in Dhaka, Bangladesh.
Nure Alam Siddiky,Samun Sarker,Md. Shahidur Rahman Khan,Ruhena Begum,Md. Ehsanul Kabir,Md. Rezaul Karim,Md. Rezaul Karim,Md. Tanvir Rahman,Asheak Mahmud,Mohammed A. Samad +9 more
- 28 Apr 2021
TL;DR: In this paper, the authors identify the pathogenicity and antimicrobial resistance (AMR) profiles of Salmonellaenterica serovars recovered from chicken at wet markets in Bangladesh.
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Diversification of the Salmonella Fimbriae: A Model of Macro- and Microevolution
Min Yue,Shelley C. Rankin,Ryan T. Blanchet,James Nulton,Robert Edwards,Robert Edwards,Dieter M. Schifferli +6 more
TL;DR: Allelic variation among adhesins attested to the pathoadaptive evolution of Salmonella towards specific hosts and tissues, potentially modulating host range, strain virulence, disease progression, and transmission efficiency.
A loop-mediated isothermal amplification method targets the phoP gene for the detection of Salmonella in food samples.
Xuefei Li,Shu Zhang,Hongwei Zhang,Lihuai Zhang,Haitao Tao,Jia Yu,Wenjie Zheng,Chenghu Liu,Dan Lu,Rong Xiang,Yin Liu +10 more
TL;DR: The LAMP described here has the potential to become a standardized method for the rapid detection of Salmonella in diagnostic laboratories once further validated by inter-laboratory studies.
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Loop-mediated isothermal amplification for the rapid, sensitive, and specific detection of the O9 group of Salmonella in chickens.
Masashi Okamura,Yousuke Ohba,Shuichi Kikuchi,Akiko Suzuki,Hajime Tachizaki,Kazuaki Takehara,Masanari Ikedo,Tadashi Kojima,Masayuki Nakamura +8 more
TL;DR: The loop-mediated isothermal amplification (LAMP) assay developed in the present study is an effective method for the specific detection of the O9 group Salmonella serovars, including SE.
64
A Survey for Salmonella by Drag Swabbing Manure Piles in California Egg Ranches
TL;DR: This study found SE to be rare in California egg ranches, which implies that these ranches are not a major source of S. enteritidis, and typed isolates heidelberg and Salmonella cerro represented the majority of the typed isolate.
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Real-Time Reverse Transcriptase PCR for the Rapid and Sensitive Detection of Salmonella Typhimurium from Pork
TL;DR: This robust rt-RT-PCR detects and confirms Salmonella in pork within approximately 24 h and thus is significantly faster than traditional methods that take >/=1 week and shows promise for routine testing and monitoring of Salmoneella by the pork industry.
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