Dedifferentiation alters chondrocyte nuclear mechanics during in vitro culture and expansion

TL;DR: In this paper , a lacunar hyaluronic acid microcarriers (LHAMC) with mechanotransductive conditions were designed to rapidly form hyaline cartilaginous microtissue regeneration.

TL;DR: In this article , the authors identify a threshold to mechanical priming separating reversible and irreversible effects of mechanical memory following 2D cell expansion, and reveal the therapeutic potential of inhibitors of epigenetic modifiers as disruptors of mechanical memories when large numbers of phenotypically suitable cells are required for regeneration procedures.

TL;DR: In this article , the secondary structure of gelatin is examined and the possible correlations between structure, mechanical and biological features is reported, and a positive correlation between failure strain and helix conformation and a negative correlation with β-structures is found.

TL;DR: In this article , a drop-based microfluidics is used to culture single chondrocytes in alginate microgels for cell-directed pericellular matrix synthesis that closely mimics the in vivo microenvironment.

TL;DR: In this article, the authors quantify the viscoelastic properties of mechanically and chemically isolated nuclei of articular chondrocytes using micropipet aspiration in conjunction with a theoretical visco-elastic model.

TL;DR: It is suggested that local stresses applied to integrins propagate from the tensed actin cytoskeleton to the LINC complex and then through lamina-chromatin interactions to directly stretch chromatin and upregulate transcription.

TL;DR: It is shown that mechanical stretch deforms the nucleus, which cells initially counteract via a calcium-dependent nuclear softening driven by loss of H3K9me3-marked heterochromatin.

TL;DR: For redifferentiation of dedifferentiated HACs, 3D cultures exhibit the most potent chondrogenic potential, whereas a hypertrophic phenotype is best achieved in 2D cultures.